GC-MS结合保留指数法分析南天竹叶、果挥发油成分  

Analysis of volatile oil in leaves and fruits of Nandina by GC-MS combined with retention index method

作　　者：王延云[1] 胡强[1,2] 杨春林 王燕 苏泽[1] WANG Yanyun;HU Qiang;YANG Chunlin;WANG Yan;SU Ze(College of Life Science,Leshan Normal University,Leshan 614000;Bamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province,Leshan Normal University,Leshan 614000;Leshan Plant Protection and Quarantine Station of Sichuan Province,Leshan 614000)

机构地区：[1]乐山师范学院生命科学学院,乐山614000 [2]乐山师范学院竹类病虫防控与资源开发四川省重点实验室,乐山614000 [3]四川省乐山市植保植检站,乐山614000

出　　处：《中国食品添加剂》2022年第6期197-207,共11页China Food Additives

基　　金：乐山市科技局重点研究项目(20NZD006)。

摘　　要：采用水蒸气蒸馏法提取南天竹叶、果挥发油成分,并通过气相色谱-质谱联用(GC-MS)结合保留指数(RI)法对其定性,峰面积归一法对其定量,分析南天竹叶、果挥发油成分含量及组成。结果显示:南天竹叶、果挥发油成分中分别鉴定出88和82种化合物,其中南天竹叶挥发油主要成分为叶绿醇(18.94%)和植酮(12.79%),并含有44种特有化合物;南天竹果挥发油主要成分为间二甲苯(28.06%)、棕榈酸(11.58%)和邻二甲苯(10.13%),并含有20种特有化合物。南天竹叶挥发油以醇类化合物(28.58%)、酮类化合物(24.80%)和苯类化合物(9.72%)为主;南天竹果挥发油以苯类化合物(48.97%)、酯类化合物(23.10%)和酸类化合物(11.68%)为主。可见,南天竹叶、果挥发油成分含量及种类具有明显差异。The volatile oil from leaves and fruits of Nandina was extracted by steam distillation,it was identified by gas chromatography-mass spectrometry(GC-MS)combined with retention index(RI)method,and quantified by peak area normalization method.Then,the content and composition of volatile oil in leaves and fruits of Nandina were analyzed.The results showed that 88 and 82 compounds were identified in the volatile oil from leaves and fruits,respectively.Among them,the main components in the volatile oil of Nandina leaves are phytol(18.94%)and phytone(12.79%),and 44 kinds of special compounds.The main components in the volatile oil of Nandina fruits were m-xylene(28.06%),palmitic acid(11.58%)and o-xylene(10.13%),and there were 20 peculiar compounds.The main volatile oil of Nandina leaves were alcohols(28.58%),ketones(24.80%)and benzenes(9.72%).Benzene compounds(48.97%),ester compounds(23.10%)and acid compounds(11.68%)were the main volatile oil of Nandina fruits.It can be seen that the content and species of volatile oil in leaves and fruits of Nandina are obviously different.

关 键 词：南天竹叶 南天竹果 挥发油 气相色谱-质谱 保留指数 

分 类 号：TS202.3[轻工技术与工程—食品科学] O657.71[轻工技术与工程—食品科学与工程]