复合纤维素酶对小麦麸降解条件的优化  

Optimization of degradation conditions of wheat bran by compound cellulase

作　　者：索江华[1] 唐桂芬[1] 黄锋 李新峰 张晓静[1] SUO Jianghua;TANG Guifen;HUANG Feng;LI Xinfeng;ZHANG Xiaojing(College of Food and Bioengineering,Henan University of Animal Husbandry and Econory,Zhengzhou Henan 450046;Henan Yongcheng pasturage bureau,Yongcheng Henan 476610)

机构地区：[1]河南牧业经济学院食品与生物工程学院,河南郑州450046 [2]河南省永城市畜牧局,河南永城476610

出　　处：《现代牧业》2022年第2期19-24,共6页Modern Animal Husbandry

基　　金：河南省科技攻关计划项目(192102110075,222102110156)。

摘　　要：为了提高复合纤维素酶对小麦麸中纤维素的降解效率,以还原糖的生成量为指标,在影响纤维素酶降解效率的单因素研究基础上,应用响应面分析进一步优化反应条件。获得的125 U/mL复合纤维素酶在48℃、pH值为4.8时降解小麦麸18 h,还原糖含量的平均值为114.31 mg/L,与未加复合酶的小麦麸还原糖相比提高了137%,有效地提高了糖化率。In order to improve the degradation efficiency of compound cellulase on cellulose in wheat bran,response surface analysis was applied to further optimize the reaction conditions based on the single factor study of reducing sugar production as index,and 125 U/mL compound cellulase was obtained.At 48 h pH value 4.8,the average reducing sugar content of wheat bran degraded for 18 h was 114.31 mg/L,which was 58%higher than that of wheat bran degraded without compound enzyme,effectively improving the saccharification rate.

关 键 词：纤维素酶 小麦麸 响应面分析 优化 

分 类 号：TS262.2[轻工技术与工程—发酵工程]