筛选出芽短梗霉生产低聚果糖探究溶氧的影响  

Screening Aureobasidium pullulans for Producing Fructo-Oligofructoses and Exploring the Effect of Dissolved Oxygen

作　　者：李成林 曹伟锋[2] 秦林莉 刘郅骞 张静 刘思伽 竹源 梁欣泉[1] LI Cheng-lin;CAO Wei-feng;QIN Lin-li;LIU Zhi-qian;ZHANG Jing;LIU Si-jia;ZHU Yuan;LIANG Xin-quan(School of Light Industry and Food Engineering,Guangxi University,Nanning 530004,China;Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100089,China)

机构地区：[1]广西大学轻工与食品工程学院,南宁530004 [2]中国科学院过程工程研究所,北京100089

出　　处：《中国调味品》2022年第7期24-30,共7页China Condiment

基　　金：中国科学院科技服务网络计划(KFJ-STS-QYZX-096)。

摘　　要：目的:从土壤树叶中筛选出芽短梗霉菌和实验室已有的出芽短梗霉,探究出芽短梗霉菌产低聚果糖的发酵性能。方法:对筛选的出芽短梗霉菌和实验室保存的出芽短梗霉菌进行发酵,通过高效液相色谱法测定得出最佳菌株,改变溶氧发酵条件探究对发酵产低聚果糖的影响。结果:通过筛选和发酵,筛选出产低聚果糖最高的出芽短梗霉菌IPE-3。结论:IPE-3是一种优秀的产低聚果糖的菌株,在600 r/min、1.8 L/min通气量和0 bar条件下,低聚果糖的产量高。Objective:To screen out Aureobasidium pullulans from soil leaves and Aureobasidium pullulans that has existed in the laboratory,and explore the fermentation performance of fructo-oligofructoses produced by Aureobasidium pullulans.Method:Ferment the selected Aureobasidium pullulans and laboratory-preserved Aureobasidium pullulans.The best strains are determined by high performance liquid chromatography,and the dissolved oxygen fermentation conditions are changed to explore the effect on fructo-oligofructoses produced through fermentation.Results:Through screening and fermentation,Aureobasidium pullulans IPE-3 which produces the most fructo-oligosaccharides is screened.Conclusion:IPE-3 is an excellent fructo-oligosaccharide-producing strain,under the conditions of 600 r/min,1.8 L/min ventilation volume and 0 bar,the yield of fructo-oligosaccharides is high.

关 键 词：出芽短梗霉菌 低聚果糖 筛选 优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]