米糠多糖提取工艺优化及抗氧化活性研究  

Optimization of extraction of polysaccharide from rice bran and its antioxidant activity

作　　者：李雪晖[1] 田龙[1] 罗心雨 王艺 王莹 LI Xue-hui;TIAN Long;LUO Xin-yu;WANG Yi;WANG Ying(School of Life Science and Agricultural Engineering,Nanyang Normal University,Nanyang 473061,Henan,China;Institute of Eco-environmental and Soil Sciences,Guangdong Academy of Sciences,Guangzhou 510650,Guangdong,China)

机构地区：[1]南阳师范学院生命科学与农业工程学院,河南南阳473061 [2]广东省生态环境技术研究所,广东广州510650

出　　处：《粮食与油脂》2022年第6期120-123,130,共5页Cereals & Oils

基　　金：国家级大学生创新创业训练计划项目(202110481003X);教育部产学合作协同育人项目(202101121018、202101199062);广东省科学院平台建设专项项目(:2019GDASYL-0102005)。

摘　　要：以米糠为原料提取多糖,在单因素试验的基础上,利用响应面分析法优化多糖提取工艺,并建立回归模型,同时探究其体外抗氧化活性。结果表明:米糠多糖的最佳提取工艺为提取温度85℃,提取时间3 h,乙醇体积分数80%,液料比25∶1(mL/g),在此条件下,多糖得率为17.31%。提取的米糠多糖对·OH、DPPH·和O_(2)^(-)·清除作用明显,因此其具有较高的抗氧化活性,是一种良好的天然抗氧化剂,且其质量浓度与抗氧化活性呈现一定的量效关系。With rice bran as raw material,on the basis of single factor experiment,the polysaccharide extraction process was optimized by response surface analysis.The regression model was established to explore its in vitro antioxidant activity.The results showed that the optimum extraction conditions were as follows:extraction temperature 85℃,extraction time 3 h,ethanol final volume fraction 80%,liquid/solid ratio 25∶1(mL/g).Under these coditions,the yield of polysaccharide was 17.31%.The extracted rice bran polysaccharide had obvious scavenging effect on·OH,DPPH·and O_(2)^(-)·,so it had high antioxidant activity and was a good natural antioxidant,and its mass concentration had a certain dose-effect relationship with antioxidant activity.

关 键 词：多糖 响应面 工艺优化 抗氧化 

分 类 号：TS201.2[轻工技术与工程—食品科学]