Distinct neuronal excitability alterations of medial prefrontal cortex in early-life neglect model of rats  

作　　者：Yu Zhang Xiuping Sun Changsong Dou Xianglei Li Ling Zhang Chuan Qin 

机构地区：[1]NHC Key Laboratory of Human Disease Comparative Medicine,Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences(CAMS),Comparative Medicine Center,Peking Union Medical College(PUMC),Beijing,China [2]National Human Diseases Animal Model Resource Center,Beijing,China [3]Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases,Beijing,China [4]International Center for Technology and Innovation of animal model,Beijing,China [5]Changping National laboratory(CPNL),Beijing,China

出　　处：《Animal Models and Experimental Medicine》2022年第3期274-280,共7页动物模型与实验医学（英文）

基　　金：CAMS Innovation Fund for Medical Sciences(CIFMS),Grant/Award Number:2021-I2M-1-034;National Natural Science Foundation of China,Grant/Award Number:31970510;Young Elite Scientist Sponsorship Program by CAST,Grant/Award Number:2019QNRC001。

摘　　要：Object:Early-life neglect has irreversible emotional effects on the central nervous system.In this work,we aimed to elucidate distinct functional neural changes in me-dial prefrontal cortex(mPFC)of model rats.Methods:Maternal separation with early weaning was used as a rat model of early-life neglect.The excitation of glutamatergic and GABAergic neurons in rat mPFC was recorded and analyzed by whole-cell patch clamp.Results:Glutamatergic and GABAergic neurons of mPFC were distinguished by typi-cal electrophysiological properties.The excitation of mPFC glutamatergic neurons was significantly increased in male groups,while the excitation of mPFC GABAergic neurons was significant in both female and male groups,but mainly in terms of rest membrane potential and amplitude,respectively.Conclusions:Glutamatergic and GABAergic neurons in medial prefrontal cortex showed different excitability changes in a rat model of early-life neglect,which can contribute to distinct mechanisms for emotional and cognitive manifestations.

关 键 词：early-life neglect model GABAERGIC GLUTAMATERGIC maternal separation with early weaning medial prefrontal cortex neuronal excitability 

分 类 号：R-332[医药卫生]