Pertussis toxin–induced inflammatory response exacerbates intracerebral haemorrhage and ischaemic stroke in mice  

作　　者：Ming Zou Yan Feng Yuwhen Xiu Yan Li Ying Zhang Junwan Fan Haowen Li Jingli Cao Wenyan He Wei-Na Jin 

机构地区：[1]Tianjin Neurological Institute,Tianjin Medical University General Hospital,Tianjin,China [2]China National Clinical Research Center for Neurological Diseases/Advanced Innovation Center for Human Brain Protection,Beijing Tiantan Hospital,Beijing,China

出　　处：《Stroke & Vascular Neurology》2022年第1期29-37,共9页卒中与血管神经病学（英文）

基　　金：supported in part by the National Science Foundation of China(81971094,81771274);the Advanced Innovation Center for Human Brain Protection,Capital Medical University,Beijing,China.

摘　　要：Background Stroke is a devastating disease,including intracerebral haemorrhage(ICH)and ischaemic stroke.Emerging evidences indicate that systemic inflammatory cascades after stroke contribute to brain damage.However,the direct effects and features of systemic inflammation on brain injury,especially comparing between ischaemic and haemorrhagic stroke,are still obscure.Methods Pertussis toxin(PT)was used to build a pro-inflammatory milieu after ICH and ischaemic stroke in mouse model.The neurodeficits,stroke lesion,immune response and blood–brain barrier(BBB)destruction were assessed.Results In ICH mouse model,PT-induced systemic inflammation exacerbated neurological deficits,and enlarged haemorrhage lesion and perihaematomal oedema.We also found promoted leucocyte infiltration and inflammatory cytokine release into the brain after PT treatment.Moreover,the integrity of the BBB was further disrupted after receiving PT.Furthermore,we demonstrated that PT enhanced brain inflammation and aggravated stroke severity in middle cerebral artery occlusion mouse model.Conclusions Our results suggest that PT increases inflammatory response that exacerbates brain injury after ICH or ischaemic stroke in mouse model.

关 键 词：INFLAMMATORY INFLAMMATION INTRACEREBRAL 

分 类 号：R516.6[医药卫生—内科学] R743.34[医药卫生—临床医学]