果蔬食品中诺如病毒污染状况及检测研究进展  

Research progress on norovirus contamination status and detection technology in fruit and vegetable foods from a non-traditional perspective

作　　者：崔健 杨莉莉 邓婷婷[2] 郑秋月 曹际娟 CUI Jian;YANG Li-Li;DENG Ting-Ting;ZHENG Qiu-Yue;CAO Ji-Juan(Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education,College of Life Science,Dalian Minzu University,Dalian 116600,China;Chinese Academy of Inspection and Quarantine,Beijing 322001,China)

机构地区：[1]大连民族大学生命科学学院,生物技术与资源利用教育部重点实验室,大连116600 [2]中国检验检疫科学研究院,北京322001

出　　处：《食品安全质量检测学报》2022年第12期3884-3892,共9页Journal of Food Safety and Quality

基　　金：国家重点研发计划项目(2021YFF0601902);辽宁省“兴辽英才计划”项目(XLYC2002106)。

摘　　要：食源性疾病不但严重危害人们的健康,而且造成大量的经济损失。在全球范围内与果蔬等农产品相关的诺如病毒(norovirus,NoV)污染事件持续暴发并呈增长态势。由于果蔬类食品中诺如病毒污染含量较低,再加上食品基质成分的干扰,使得果蔬制品中的NoV检测成为一项非常艰巨的任务。传统的检测技术存在许多缺点,例如鉴定的特异性有待增强;提取效率不够高,检测敏感性尚需改进。这意味着需要转换视角,开展食源性诺如病毒检测技术的创新研究。本文综述了果蔬食品中NoV污染流行状况及易受污染的、需加以关注的果蔬食品种类,总结了果蔬食品中NoV洗脱及富集方法、介绍了基于组学的检测技术及发展趋势,为不断提高果蔬食品中NoV检测防控能力提供参考。Foodborne virus diseases not only seriously endanger people’s health,but also cause a lot of economic losses.Worldwide,norovirus(NoV)pollution incidents related to fruits and vegetables and other agricultural products continue to break out and show an increasing trend.Due to the low content of norovirus contamination in fruit and vegetable foods,coupled with the interference of food matrix components,NoV detection in fruit and vegetable products has become a very difficult task.The traditional detection technology exist many shortcomings,for instance,the specificity of identification needs to be enhanced;extraction efficiency is not high enough,and detection sensitivity needs to be continuously improved.This means that it is necessary to change the perspective and carry out innovative research on detection technology of foodborne norovirus.This paper summarized the prevalence of NoV pollution in fruit and vegetable foods and the types of fruit and vegetable foods that were vulnerable to pollution and need attention,summarized the methods of elution and enrichment of NoV in fruits and vegetables,and introduced the detection technology and development trend based on omics,in order to provide reference for continuously improving the detection and control ability of NoV in fruits and vegetables.

关 键 词：诺如病毒 果蔬食品 污染状况 洗脱及富集 组学检测技术 

分 类 号：R155.5[医药卫生—营养与食品卫生学]