一种鬼笔属真菌分离鉴定和挥发性成分分析  

A Phallus Fungi Isolation&Identification and Analysis of Volatile Components

作　　者：易雪倩 徐宁 王小艳 黄晓辉 刘祝祥[1] YI Xueqian;XU Ning;WANG Xiaoyan;HUANG Xiaohui;LIU Zhuxiang(College of Biological Resources and Environmental Sciences,Jishou University,Jishou 416000,Hunan,China;Edible Fungi Institute of Hunan Province,Changsha 410000,Hunan,China;Luliangcuan Village Green Circle Mushroom Co.,Ltd.,Qujing 65500,Yunan,China)

机构地区：[1]吉首大学生物资源与环境科学学院,湖南吉首416000 [2]湖南省食用菌研究所,湖南长沙410000 [3]陆良爨乡绿圆菇业有限公司,云南曲靖655000

出　　处：《中国蔬菜》2022年第7期78-84,共7页China Vegetables

基　　金：云南省科技计划项目(202002AE320003-03-03);湖南省重点研发计划项目(2020NK2053)。

摘　　要：通过传统形态分类学和分子系统学方法对采自湖南西部的野生大型真菌进行鉴定,采用固相微萃取-气相色谱-质谱联用(SPME-GC-MS)技术分析真菌子实体菌盖的挥发性成分。结果表明:该野生大型真菌鉴定为鬼笔属的冬荪(Phallus dongsun),属于新鉴定的种,子实体白色蕾球形,菌托淡黄色苞状,菌盖钟形淡黄色,附有棕黑色的孢子液,带有黏性和特殊气味,孢子大小为(1.80~2.31)μm×(3.70~4.58)μm,杆状,无色。从冬荪子实体菌盖中共鉴定出72种挥发性成分,主要为苯甲醚、邻苯二甲醚、苯乙醇、对苯二甲醚,其中苯甲醚相对含量最高,为35.513%;香气成分23种,占总成分的72.805%,含量较高的是苯甲醚、苯乙醇、对苯二甲醚、苯乙醛和正戊醇。This paper conducted identification on wild microfungi collected from west part of Hunan by tranditional morphological taxonom and molecular systematics methods;analyzed the volatile components of fungus fruiting body pilelus by SPME-GC-MS technology.Results showed that this wild microfungi fungi was identified as Phallus dongsun belonging to a newly identified species.The fruiting body was white in color and of spherical shape.The teleblem(volva)was yellowish in color and of bud shape.The pileus was also yellowish in color and of bell shape with brown-black spore liquid,sticky and special smell.The spore size was(1.80-2.31μm)×(3.70-4.58μm).Spore was of rod shape,and colorless.72 volatile components were identified from pileus of Phallus dongsun.The main components were anisole,phthalyl alcohol,phenethyl alcohol,para-dimethyl ether,of which the relative content of anisole was the highest(35.513%).There were 23 kinds of aroma component,accounting for 72.805%of the total composition.Those with higher contents were anisole,phenethyl alcohol,para-dimethyl ether,phenylacetaldehyde and n-amyl alcohol.

关 键 词：冬荪 鬼笔属 SPME-GC-MS 挥发性成分 香气成分 

分 类 号：O657.63[理学—分析化学] TS219[理学—化学]