山茱萸多糖提取工艺优化及对α-葡萄糖苷酶的抑制作用  

Optimization of Extraction Technology of Polysaccharides from Cornus officinalis and Inhibition ofα-Glucosidase

作　　者：任俊鹏 杨志萍 陈贵堂 綦国红 REN Junpeng;YANG Zhiping;CHEN Guitang;QI Guohong(School of Engineering/National R&D Center for Chinese Herbal Medicine Processing,China Pharmaceutical University,Nanjing 211198)

机构地区：[1]中国药科大学工学院/国家中药材加工技术研发专业中心,南京211198

出　　处：《食品工业》2022年第4期4-7,共4页The Food Industry

基　　金：江苏现代农业产业技术体系建设专项(JATS[2020]413)。

摘　　要：对山茱萸粗多糖超声辅助法提取工艺进行优化并研究其在体外对α-葡萄糖苷酶的抑制作用,考察其降血糖活性的潜能。以粗多糖提取率为考察指标,通过单因素试验及正交试验对山茱萸粗多糖的提取条件进行优化并验证。通过PNPG法以阿卡波糖为阳性对照测定山茱萸粗多糖对α-葡萄糖苷酶的抑制作用。结果表明,山茱萸粗多糖提取最佳条件为超声温度60℃、超声功率450 W、超声时间50 min、料液比1︰30 g/mL。在此条件下,山茱萸粗多糖的提取率为30.43%。10 mg/mL的山茱萸粗多糖对α-葡萄糖苷酶活性的抑制率达70%,具有降血糖活性的潜能。Aim to optimize the ultrasonic-assisted extraction method of crude polysaccharide from Cornus officinalis.Its inhibition effect on α-glucosidase in vitro as well as its potential of hypoglycemic activity was studied.Single factor test and orthogonal test were performed to optimize the extraction condition of the crude polysaccharide using extraction efficiency as index.Then the inhibition ofα-glucosidase by extracted crude polysaccharide was determined by PNPG method with acarbose as the positive control.The results showed that the optimum condition was temperature 60℃,power 450 W,time 50 min,and material-liquid ratio of 1︰30 g/mL.The extraction efficiency of crude polysaccharide was 30.43% under this condition.The inhibition ratio of α-glucosidase by 10 mg/mL crude polysaccharide was 70%.It is suspected that crude polysaccharide from Cornus officinalis might have a potential hypoglycemic effect.

关 键 词：山茱萸 粗多糖 正交设计 Α-葡萄糖苷酶 

分 类 号：R284.2[医药卫生—中药学]