米曲霉发酵提取甘薯中去氢表雄酮工艺优化  

Optimization of Extraction Process of Dehydroepiandrosterone from Sweet Potato through Aspergillus oryzae Fermentation

作　　者：陈蓬凤 邹浩峰 蔡芳[1] 隋勇[1] 施建斌[1] 蔡沙[1] 熊添 陈学玲[1] 黄师荣[2] 梅新[1] CHEN Peng-feng;ZOU Hao-feng;CAI Fang;SUI Yong;SHI Jian-bin;CAI Sha;XIONG Tian;CHEN Xue-ling;HUANG Shi-rong;MEI Xin(Institute of Agro-Product Processing and Nuclear-Agricultural Technology,Hubei Academy of Agricultural Science,Wuhan 430064,Hubei,China;College of Chemical Engineering,Xiangtan University,Xiangtan 411105,Hunan,China)

机构地区：[1]湖北省农业科学院农产品加工与核农技术研究所,湖北武汉430064 [2]湘潭大学化工学院,湖南湘潭411105

出　　处：《食品研究与开发》2022年第14期165-172,共8页Food Research and Development

基　　金：湖北省技术创新专项(2019AEE022)。

摘　　要：以甘薯生全粉为原料,采用米曲酶发酵提取去氢表雄酮(dehydroepiandrosterone,DHEA),基于提取物中DHEA定性分析基础上,探讨米曲酶孢子悬浮液接种量、发酵时间、发酵温度和液固比等因素对米曲霉发酵提取甘薯中DHEA得率的影响,结合单因素与响应面试验优化得最优发酵工艺:米曲酶孢子悬浮液接种量6%,发酵时间72 h,发酵温度30℃,液固比15∶1(mL/g),此条件下DHEA得率可达94.70μg/100 g,显著(p<0.05)高于预发酵-酸解法、酶解法提取DHEA得率。Using whole sweet potato powder as the raw material and on the basis of pre-testing the identification of the sweet potato fermentation extract,four factors(fermentation liquid ratio,fermentation time,inoculum amount,and fermentation temperature)were studied in the extraction of dehydroepiandrosterone(DHEA)from sweet potato through fermentation using Aspergillus oryzae.Combined with response surface analysis,the optimal technological conditions for extracting DHEA from sweet potato by Aspergillus oryzae fermentation were revealed as:fermentation material to liquid ratio of 1∶15(g/mL),fermentation time 72 h,inoculum 6%,and fermentation temperature 30℃.Under these conditions,the DHEA yield reached 94.70μg/100 g,which was close to the theoretical prediction value of 97.07μg/100 g.Compared with the existing pre-fermentation-acid hydrolysis method,enzymatic hydrolysis significantly increased DHEA yield(p<0.05).

关 键 词：去氢表雄酮 米曲霉 发酵 提取 响应面分析 

分 类 号：TS215[轻工技术与工程—粮食、油脂及植物蛋白工程]