HPLC-ELSD法测定天然香料中水溶性糖的分布特征  

Distribution characteristics of soluble sugars in natural fragrance and flavor ingredient by HPLC-ELSD

作　　者：刘雨 席辉[1] 付英杰 李鹏[1] 卢斌斌[1] 孙世豪[1] 宗永立[1] LIU Yu;XI Hui;FU Yingjie;LI Peng;LU Binbin;SUN Shihao;ZONG Yongli(Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China)

机构地区：[1]中国烟草总公司郑州烟草研究院,河南郑州450001

出　　处：《河南工业大学学报（自然科学版）》2022年第3期89-95,共7页Journal of Henan University of Technology：Natural Science Edition

基　　金：国家烟草专卖局科技重大专项(110202001004(YX-04),110202001002(YX-02))。

摘　　要：为了研究天然香料中水溶性糖的分布特征,建立了果糖、葡萄糖、麦芽糖、麦芽三糖、肌糖醇、阿拉伯糖醇、山梨糖醇和蔗糖同步分析的高效液相色谱-蒸发光散射(HPLC-ELSD)方法,并对不同品类和原料种类的天然香料进行考察。结果表明:该方法分析性能良好,检出限低(LOD<5.00μg/mL),重现性好(RSD≤4.65%),回收率高(91.5%~98.9%);浓缩物、浸膏和提取物中果糖、葡萄糖含量均高于酊剂、净油和精油;以水果为原料的天然香料中葡萄糖和果糖含量均高于以花叶为原料的天然香料。不同品类和原料种类的天然香料中水溶性糖分布具有规律性差异,对于天然香料品质评价和实际应用具有指导性意义,同时为建立天然香料液相色谱指纹图谱真实性鉴别技术奠定了基础。In order to study the distribution characteristics of soluble sugars in natural fragrance and flavor ingredient(NFFI),an HPLC-ELSD method for simultaneous determination of fructose,glucose,maltose,maltose tritose,arabitol,inositol,sorbitol and sucrose was developed,and the method was used to investigate NFFI of different species and raw materials.The results showed that the method performed detection limit(LOD<5.00μg/mL),good reproducibility(RSD≤4.65%)and high recovery rate(92.5%-98.9%).The coefficients of variation and variation of soluble sugars in NFFI were high and the dispersion degree was large,which indicated that the distribution of soluble sugars in NFFI was obviously different.In addition,the most frequently detected soluble sugars in NFFI were glucose and fructose,followed by maltose,arabinol,sucrose,sorbitol,and maltose tritose.In addition,arabinol was only detected in tree moss extract and tree moss concrete,and inositol was only detected in chicory concrete.The content of fructose and glucose in concentrate,extract and concrete was higher than those in tincture,net oil,and essential oil.The content of fructose and glucose in the extract and concrete was significantly higher than that in the tincture.Taking the content of 8 soluble sugars in natural spices as variables,different types of NFFI can be divided into three categories,with concentrate,most extracts and some concretes were in the first category;essential oil,purified oil,tincture,partial extract and partial extract were in second category;tree moss extract and tree moss concrete were in the third category.The content of glucose and fructose in NFFI made from fruit was higher than that made from flower and leaf.The content of fructose and glucose in the concretes and extracts from fruit was significantly higher than that in the tinctures,but there was no significant difference in the content of fructose and glucose in the extracts and concretes.In addition,there was no significant difference in the content of fructose and glucose in the c

关 键 词：天然香料 高效液相色谱-蒸发光散射(HPLC-ELSD) 水溶性糖 分布特征 

分 类 号：TS201.2[轻工技术与工程—食品科学]