响应面优化沙棘果渣膳食纤维提取工艺及应用性质评价  

Extraction technology optimization of dietary fiber from Hippophae rhamnoides pomace by response surface methodology and the evaluation of its application properties

作　　者：郭京京 李雅轩 樊子怡 陈炫彤 王琪[1] 符群[1,2] GUO Jing-jing;LI Ya-xuan;FAN Zi-yi;CHEN Xuan-tong;WANG Qi;FU Qun(School of Forestry,Northeast Forestry University;Key Laboratory of Forest Food Resources Utilization of Heilongjiang Province,Harbin 150040,China)

机构地区：[1]东北林业大学林学院 [2]黑龙江省森林食品资源利用重点实验室,哈尔滨150040

出　　处：《天然产物研究与开发》2022年第7期1181-1188,共8页Natural Product Research and Development

基　　金：“十三五”国家重点研发计划(2016YFC0500307-07);国家级大学生创新项目(202110225151)。

摘　　要：为实现林下资源加工废弃物再利用,本实验以沙棘果渣为原料,采用超声辅助酶法,分别对纤维素酶添加量、超声温度、超声时间、料液比四个因素进行膳食纤维提取效果影响分析,以可溶性膳食纤维(soluble dietary fiber,SDF)和不可溶性膳食纤维(insoluble dietary fiber,IDF)得率比值为指标,通过响应面法对主要参数进行优化,并分析所获膳食纤维应用性质。结果得出,在最优条件纤维素酶添加量为5%,60℃下超声50 min时,SDF/IDF为0.21±0.01,总膳食纤维(total dietary fiber,TDF)溶胀力和持油力较高,分别为3.12±0.13 mL/g、(4.47±0.41)%,且对胆固醇的吸附效果良好。因此,超声辅助酶法能有效提高产物提取效率,对推进沙棘副产物资源的开发利用具有一定意义。In order to reuse the processing waste of forest resources,this experiment used Hippophae rhamnoides pomace as raw material,using ultrasonic-assisted enzyme method,and analyzed the effects of four factors,namely cellulase addition,ultrasonic temperature,ultrasonic time and material liquid ratio,on the extraction effect of dietary fiber.With the yield ratio of soluble dietary fiber(SDF)and insoluble dietary fiber(IDF)as the index,response surface methodology was used to analyze the extraction effect of dietary fiber.The results showed that the SDF/IDF was 0.21±0.01,and the swelling capacity and oil holding capacity of total dietary fiber(TDF)were 3.12±0.13 mL/g,(4.47±0.41)%respectively under the optimum conditions of 5%cellulase and ultrasonic treatment at 60℃for 50 min.Therefore,ultrasonic-assisted enzyme method can effectively improve the extraction efficiency of products,and has certain significance for promoting the development and utilization of Hippophae rhamnoide by-product resources.

关 键 词：沙棘果渣 膳食纤维 响应面 得率比值 应用性质 

分 类 号：TS2552[轻工技术与工程—农产品加工及贮藏工程]