葛根降血压茶的制备及对自发性高血压大鼠的降压作用  

Preparation of pueraria hypotensive tea by fermentation and its hypertensive effect on spontaneously hypertensive rats

作　　者：刘璐[1] 袁亚宏[1] 岳田利[1,2] LIU Lu;YUAN Ya-hong;YUE Tian-li(College of Food Science and Engineering,Northwest A&F University,Xianyang,Shaanxi 712100,China;College of Food Science and Technology,Northwest University,Xi'an,Shaanxi 710069,China)

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西咸阳712100 [2]西北大学食品科学与工程学院,陕西西安710069

出　　处：《食品与机械》2022年第7期213-219,共7页Food and Machinery

基　　金：陕西省农业科技创新项目(编号:2018DZXM-NY-084)。

摘　　要：目的:制备具有较高血管紧张素转换酶(ACE)活性体外抑制率的冠突散囊菌发酵茶,并考察发酵茶液对自发性高血压大鼠(SHR)的急性降压作用。方法:考察葛根添加量、基质含水量和孢子悬浮液对ACE酶活性体外抑制率的影响,并采用正交试验进行发酵工艺优化;水提法制备发酵茶液,选择SHR模型研究发酵茶的降血压作用。结果:最优发酵工艺为20 g绿茶中添加2 g葛根量,20%基质含水量,5%孢子悬浮液接种量。与对照组相比,不同剂量的葛根降血压茶对SHR在24 h内均有降低收缩压和舒张压作用。结论:300~600 mg/kg日用剂量的葛根降血压茶的急性降压效果最显著,可在短期内降低SHR血压。Objective:This study aimed to obtain a novel fermented green tea with high ACE-inhibitory activity by Eurotium cristatum and to investigate its acute hypotensive effects on spontaneously hypertensive rats(SHR).Methods:The effects of pueraria addition,water content of substrate and spore suspension on ACE-inhibitory activity in vitro were investigated,and the fermentation process was optimized by orthogonal design experiment.The fermented tea solution was prepared by water extraction method,and SHR model was selected to study the hypotensive effect of fermented tea.Results:The optimal fermentation process for fermented tea was as follows:adding 2 g pueraria into 20 g green tea matrix,controlling the water content of the matrix to 20%,inoculatin 5%suspension of E.cristatum.Compared with the control group,different doses of fermented tea had lowering effects on blood pressure in SHR within 24 h.Conclusion:The acute hypotensive effect of the daily dose of 300~600 mg/kg was the most significant,which could lower the blood pressure of SHR in a short period of time.

关 键 词：冠突散囊菌 葛根 绿茶 血管紧张素转换酶 自发性高血压大鼠 

分 类 号：TS272.55[农业科学—茶叶生产加工]