基于酪胺信号放大技术的新型ELISA法高灵敏检测达氟沙星  

Development of novel ELISA based on tyramine signal amplification for sensitive detection of danofloxacin

作　　者：鲍欢欢 刘成伟[2] 刘道峰[2] 赖卫华 BAO Huanhuan;LIU Chengwei;LIU Daofeng;LAI Weihua(College of Food Science and Technology,Nanchang University,Jiangxi Nanchang 330029,China;Jiangxi Province Key Laboratory of Diagnosing and Tracing of Foodborne Disease,Jiangxi Province Centre for Disease Control and Prevention,Jiangxi Nanchang 330029,China)

机构地区：[1]南昌大学食品学院,江西南昌330029 [2]江西省疾病预防控制中心/江西省食源性疾病诊断溯源重点实验室,江西南昌330029

出　　处：《中国食品卫生杂志》2022年第3期517-523,共7页Chinese Journal of Food Hygiene

基　　金：国家重点研发计划(2019YFC1605502)。

摘　　要：目的建立一种结果可视的新型ELISA法高灵敏检测达氟沙星。方法基于酪胺信号放大原理,设计一种高灵敏检测达氟沙星的新型ELISA法;通过灵敏度、特异性实验评估本方法的有效性;将本方法应用于鸡肉和猪肉加标样本的检测进行方法验证。结果本方法裸眼检测灵敏度为0.4 ng/mL,相较于传统ELISA方法(10 ng/mL)的裸眼检测灵敏度提高了25倍;在缓冲液中与其他几种常用药物无明显交叉反应;对鸡肉和猪肉样本中加标的达氟沙星可有效检出。结论本方法灵敏度高,特异性好,可用于实际样本中达氟沙星的现场裸眼定性检测。Objective To develop a novel ELISA for sensitive and naked-eye detection of danofloxacin(DAN).Methods Based on TYR signal amplification,a novel ELISA was developed for sensitive detection of DAN.Analytical performance of the method was conducted by sensitivity and specificity experiments.The method was used for detecting DAN-spiked chicken and pork samples to verify.Results This method could be used to qualitatively detect DAN with naked eyes at a cut-off value of 0.4 ng/mL,which was 25-fold lower than that of the traditional ELISA(10 ng/mL).The method exhibited no cross-reaction of DAN with six other drugs in buffer.The method was successfully used for detecting DAN-spiked chicken and pork samples.Conclusion The developed novel ELISA exhibits high sensitivity and specificity,and can be used for the danofloxacin detection in actual samples by naked eye.

关 键 词：酪胺信号放大技术 胶体金 抗坏血酸 达氟沙星 

分 类 号：R155[医药卫生—营养与食品卫生学]