CD301b^(+) macrophages mediate angiogenesis of calcium phosphate bioceramics by CaN/NFATc1/VEGF axis  

作　　者：Jiaolong Wang Qin Zhao Liangliang Fu Shihang Zheng Can Wang Litian Han Zijian Gong Ziming Wang Hua Tang Yufeng Zhang 

机构地区：[1]The State Key Laboratory Breeding Base of Basic Science of Stomatology(Hubei-MOST)and Key Laboratory of Oral Biomedicine,Ministry of Education,School&Hospital of Stomatology,Wuhan University,Wuhan,430079,Hubei,PR China [2]Institute of Infection and Immunity,Science and Technology Innovation Center,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan,250000,Shandong,PR China [3]Medical Research Institute,School of Medicine,Wuhan University,Wuhan,430071,Hubei,PR China

出　　处：《Bioactive Materials》2022年第9期446-455,共10页生物活性材料（英文）

基　　金：supported by the National Natural Science Foundation of China(82025011 and 82100975);the China National Postdoctoral Program for Innovative Talents(BX2021227);the Young Elite Scientist Support Program by CSA(2020PYRC001);the Fundamental Research Funds for the Central Universities(2042021kf0181 and 2042020kf0204).

摘　　要：Calcium phosphate(CaP)bioceramics are important for tissue regeneration and immune response,yet how CaP bioceramics influence these biological processes remains unclear.Recently,the role of immune cells in biomaterial-mediated regeneration,especially macrophages,has been well concerned.CD301b^(+)macrophages were a new subset of macrophages we have discovered,which were required for bioceramics-mediated bone regeneration.Nevertheless,the impact of CD301b^(+)macrophages on angiogenesis,which is a vital prerequisite to bone formation is yet indistinct.Herein,we found that CD301b^(+)macrophages were closely correlated to angiogenesis of CaP bioceramics.Additionally,depletion of CD301b^(+)macrophages led to the failure of angiogenesis.We showed that store-operated Ca^(2+)entry and calcineurin signals regulated the VEGF expression of CD301b^(+)macrophages via the NFATc1/VEGF axis.Inhibition of calcineurin effectively impaired angiogenesis via decreasing the infiltration of CD301b^(+)macrophages.These findings provided a potential immunomodulatory strategy to optimize the integration of angiogenesis and bone tissue engineering scaffold materials.

关 键 词：Calcium phosphate ceramics MACROPHAGE ANGIOGENESIS CD301b CALCINEURIN 

分 类 号：R318[医药卫生—生物医学工程]