小球藻胞内多糖提取纯化及其抗氧化活性  

Extraction and Purification of Intracellular Polysaccharide from Chlorella vulgaris and Its Antioxidant Activity

作　　者：李思雨 刘红全 孙寒 徐琰杰 黄磊恒 龙寒 凌宏林 成江弈 杨堃峰[2] LI Siyu;LIU Hongquan;SUN Han;XU Yanjie;HUANG Leiheng;LONG Han;LING Honglin;CHENG Jiangyi;YANG Kunfeng(Guangxi Key Laboratory of Polysaccharide Materials and Their Modification,Key Laboratory of Protection and Utilization of Marine Resources,College of Marine and Biotechnology,Guangxi University for Nationalities,Nanning 530007,China;Ocean University of China College of Marine Life Sciences,Qingdao 266003,China)

机构地区：[1]广西民族大学海洋与生物技术学院,广西多糖材料与改性重点实验室,广西民族大学海洋生物资源保护与利用重点实验室,广西南宁530007 [2]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《食品工业科技》2022年第15期209-219,共11页Science and Technology of Food Industry

基　　金：广西自然科学基金项目(2018GXNSFAA294032)。

摘　　要：为提高小球藻胞内粗多糖得率,并探究小球藻胞内多糖纯化组分的抗氧化作用。本研究采取超声波破碎和热水浸提相结合的方法提取小球藻胞内粗多糖,利用响应面法进行提取条件优化,在此基础上,采用阴离子交换柱和葡聚糖凝胶柱层析对提取得到的粗多糖进行分离纯化,并进行结构表征和抗氧化试验。响应面结果显示,小球藻胞内粗多糖最优提取条件为:NaOH质量分数为2.0%,料液比为1:25(g/mL),超声功率为200 W,超声时间为20 min,提取温度为80℃,提取时间为1.5 h,在此条件下,小球藻胞内粗多糖的得率为18.086%±0.143%。结构表征和体外抗氧化结果显示,纯化多糖(Purification of intracellular polysaccharide,SCIP),主要由葡萄糖、鼠李糖及半乳糖成分组成,是一种含有糖醛酸的吡喃糖。其在20 mg/mL时,对1,1-二苯基-2-苦基肼(1,1-Diphenyl-2-picrylhydrazyl,DPPH)自由基的清除率最大,为75.64%±1.56%,在25 mg/mL时,对羟基自由基清除率最大,为71.08%±0.58%,IC分别6.42 mg/mL和8.59 mg/mL。研究结果为深入了解小球藻胞内多糖理化性质及小球藻多糖的开发利用提供基础。In order to improve the yield of crude polysaccharide from Chlorella vulgaris and explore the antioxidant effect of purified intracellular polysaccharide from Chlorella vulgaris,this study adopted the method of ultrasonic crushing and hot water extraction to extract intracellular crude polysaccharides from Chlorella vulgaris.Response surface method was used to optimize the extraction conditions.On this basis,anion exchange column and dextran gel column chromatography were used to separate and purify the extracted crude polysaccharides,and the characterization and antioxidant test were carried out.Response surface results showed that the optimal extraction conditions of intracellular crude polysaccharides from Chlorella vulgaris were as follows:The mass fraction of NaOH 2.0%,the material-liquid ratio 1:25(g/mL),the ultrasonic power 200 W,the ultrasonic time 20 min,the extraction temperature 80℃,the extraction time 1.5 h.Under these conditions,the yield of intracellular crude polysaccharide was 18.086%±0.143%.The results of characterization and antioxidant activity in vitro showed that SCIP was a pyranose sugar containing uronic acid and was composed of glucose,rhamnose and galactose.At 20 mg/mL,the DPPH radical scavenging rate was the largest of 75.64%±1.56%,and at25 mg/mL,the hydroxyl radical scavenging rate was the largest of 71.08%±0.58%.ICwere 6.42 mg/mL and 8.59 mg/mL,respectively.The results provided a basis for further understanding of the physicochemical properties and biological activities of polysaccharides from Chlorella sp.

关 键 词：小球藻 胞内多糖 提取优化 分离纯化 抗氧化 

分 类 号：TS201.2[轻工技术与工程—食品科学]