食用菌β-(1→3)-葡聚糖碱提条件优化及含量测定  

Optimization of Extraction Conditions and Content Measurement ofβ-(1→3)-Glucan in Edible Fungi

作　　者：连泽柔 刘静 张劲松[2] 唐庆九[2] 冯杰[2] 周帅[2] 刘艳芳[2] LIAN Zerou;LIU Jing;ZHANG Jingsong;TANG Qingjiu;FENG Jie;ZHOU Shuai;LIU Yanfang(China College of Food Science&Technology,Shanghai Ocean University,Shanghai 201306,China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungal Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,National Engineering Research Center of Edible Fungi,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点实验室,上海201403

出　　处：《食用菌学报》2022年第4期69-76,共8页Acta Edulis Fungi

基　　金：上海市科技兴农重点攻关项目(X2021-02-08-00-12-F 00797);上海市农业科学院卓越团队建设计划(G2022003);上海市闵行区领军人才计划(201844)。

摘　　要：采用单因素(碱液种类、碱液浓度、料液比、提取时间和温度)实验对提取刺芹侧耳(Pleurotus eryngii)水提残渣中β-(1→3)-葡聚糖条件进行优化,获得最佳提取工艺:以1 mol·L-1 KOH溶液按照料液比1∶60(g∶mL)添加,在60℃水浴条件下提取1.5 h,刺芹侧耳水提残渣β-(1→3)-葡聚糖含量为26.28%。以该条件对18种食用菌子实体、水提物和水提残渣的β-(1→3)-葡聚糖含量进行测定,结果表明:绣球菌(Sparassis crispa)、猴头菌(Hericium erinaceus)中β-(1→3)-葡聚糖含量较高,分别为24.91%和20.64%;毛头鬼伞(Coprinus comatus)、蛹虫草(Cordyceps militaris)、羊肚菌(Morchella esculenta)、姬松茸(Agaricus blazei)和黑木耳(Auricularia auricula)中含量均低于3%。不同食用菌水提物得率(4.98%~50.45%)差异较大,除灵芝(Ganoderma lucidum)外,各食用菌水提物中β(-1→3)-葡聚糖含量均较低(0.41%~4.21%)。绣球菌、猴头菌、牛肝菌(Boletus bainiugan)和刺芹侧耳水提残渣β-(1→3)-葡聚糖含量较高,均大于20%,可作为制备食用菌β-(1→3)-葡聚糖的优质原料。Using water extraction residue of Pleurotus eryngii as the material,single-factor experiments were carried out to optimizeβ-(1→3)-glucan extraction conditions,including alkali type,alkali concentration,solidliquid ratio,extraction time and extraction temperature.The results showed that the optimalβ-(1→3)-glucan extraction conditions were as follows:adding 1 mol·L-1 KOH solution at a solid-liquid ratio of 1∶60(g∶mL)and extracting in water bath at 60℃for 1.5 h.The resultant extract of P.eryngii water extraction residue contained 26.28%β-(1→3)-glucan.Then the content ofβ-(1→3)-glucan in fruiting bodies,water extracts and water extraction residues of 18 edible fungi were determined.The content ofβ-(1→3)-glucan was high in fruiting bodies of Sparassis crispa and Hericium erinaceus,reaching 24.91%and 20.64%,respectively.On the other hand,fruiting bodies of Coprinus comatus,Cordyceps militaris,Morchella esculenta,Agaricus blazei and Auricularia auricula contained<3%β-(1→3)-glucan.Water extract yields of different edible fungi varied widely,ranging from 4.98%to 50.45%.Except Ganoderma lucidum,the contentβ-(1→3)-glucan of in the water extracts of the remaining 17 edible fungi was low(0.41%-4.21%).The water extraction residues of Sparassis crispa,Hericium erinaceus,Boletus bainiugan and Pleurotus eryngii contained more than 20%β-(1→3)-glucan,and thus can be used as high-quality raw materials for preparingβ-(1→3)-glucan from edible fungi.

关 键 词：刺芹侧耳 Β-葡聚糖含量 苯胺蓝荧光法 碱液提取 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.3[轻工技术与工程—食品科学与工程]