异麦芽酮糖减少小鼠肝脏脂肪堆积的关键基因筛选与验证  

Screening and Verification of Key Genes for Isomaltulose Reducing Liver Fat Accumulation in Mice

作　　者：何秋玲 张彩平 桂静 刘泳杏 季久秀 周波 张震 HE Qiuling;ZHANG Caiping;GUI Jing;LIU Yongxing;JI Jiuxiu;ZHOU Bo;ZHANG Zhen(College of Pharmacy,Guilin Medical University,Guilin 541199,China;College of Intelligent Medicine and Biotechnology,Guilin Medical University,Guilin 541199,China;College of Agriculture and Forestry,Linyi University,Linyi 276005,China;Scientific Research Center,Guilin Medical University,Guilin 541199,China)

机构地区：[1]桂林医学院药学院,广西桂林541199 [2]桂林医学院智能医学与生物技术学院,广西桂林541199 [3]临沂大学农林科学学院,山东临沂276005 [4]桂林医学院科学实验中心,广西桂林541199

出　　处：《食品工业科技》2022年第16期1-8,共8页Science and Technology of Food Industry

基　　金：广西自然科学基金(2020GXNSFBA297026);广西高校中青年教师基础能力提升项目(2020KY12007);山东自然科学基金(ZR2019PC034);国家级大学生创新创业训练项目(202010601028)。

摘　　要：为筛选和验证异麦芽酮糖减少小鼠肝脏脂肪堆积的关键基因,本研究利用Limma软件包对异麦芽酮糖和蔗糖饲喂22周的雄性小鼠肝脏转录组数据进行差异表达分析(GSE54723,n=14),共筛选出49个DEGs,通过Metascape软件对DEGs进行GO功能富集分析和KEGG信号通路分析,使用Cytoscape软件和WGCNA软件包构建基因共表达网络,取重叠基因搜寻关键节点基因,挖掘得到10个关键节点基因Pnliprp1、Prss2、Clps、Tff2、Pnlip、Ctrl、Cpa1、Cel、Dmbt1、Vil1,基因功能分析表明Pnlip、Pnliprp1、Cel、Clps与脂质代谢相关。qPCR结果显示,异麦芽酮糖组肝脏组织中这4个与脂质代谢相关基因的表达量显著高于蔗糖组,通过比较正常人和非酒精性脂肪肝患者肝脏组织转录组数据中上述基因的表达量(GSE163211,n=318),发现正常人Pnlip、Pnliprp1、Cel、Clps基因表达量亦显著(P<0.05)高于病例组。本研究揭示了异麦芽酮糖通过上调Pnlip、Pnliprp1、Cel、Clps的合成促进脂肪分解从而降低肝脏脂质的积累,为异麦芽酮糖影响肝脏脂质代谢的分子机制研究提供了理论支撑。In order to screen and verify the key genes that isomaltulose reduces liver fat accumulation in mice, in this study,the Limma package was used to analyze the liver transcriptome data of male mice fed with isomaltose and sucrose for 22weeks(GSE54723, n=14). A total of 49 DEGs were screened out, and then were analyzed by Metascape software for GO functional enrichment analysis and KEGG signaling pathways analysis. Cytoscape and WGCNA software were used to construct gene co-expression network, overlapping genes were picked up for searching for key node genes, and 10 key node genes Pnliprp1, Prss2, Clps, Tff2, Pnlip, Ctrl, Cpa1, Cel, Dmbt1, Vil1 were screened out, gene functional analysis showed Pnlip, Pnliprp1, Cel, Clps were related to lipid metabolism. The qPCR results showed that the expression of these four genes related to lipid metabolism in liver tissue of isomaltulose group was significantly(P<0.05) higher than that of sucrose group, by comparing the expression of the above genes in the liver transcriptome data of normal people and NAFLD patients(GSE163211, n=318), Pnlip, Pnliprp1, Cel and Clps were also expressed significantly higher in normal people than in NAFLD patients. This study revealed that isomaltulose promoted lipolysis by up regulating the synthesis of Pnlip,Pnliprp1, Cel and Clps, so as to reduce the accumulation of liver lipids, which would provide a theoretical support for the study of the molecular mechanism of isomaltulose affecting liver lipid metabolism.

关 键 词：异麦芽酮糖 蔗糖 转录组数据 节点基因 脂质代谢 

分 类 号：TS241[轻工技术与工程—制糖工程]