大豆肽缓解小鼠体力疲劳的功能评价  

Evaluation on Anti-fatigue Function of Soybean Peptide in Mice

作　　者：于亚莉 蒲奕灵 高绿莎 孙冰玉 仲大业 高峰 Yu Yali;Pu Yiling;Gao Lüsha;Sun Bingyu;Zhong Daye;Gao Feng(School of Food Science and Engineering,Jilin University,Changchun 130062;Food and Materials Management Center,Siping 136000,Jilin;School of Physical,Jilin University,Changchun 130062)

机构地区：[1]吉林大学食品科学与工程学院,长春130062 [2]吉林省四平市粮食物资管理中心,吉林四平136000 [3]吉林大学体育学院,长春130062

出　　处：《中国食品学报》2022年第7期105-114,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：吉林省科技发展计划项目重大科技专项(20200502002NC);吉林省教育厅“十三五”科学技术研究规划项目(JJKH20190175KJ)。

摘　　要：评价大豆肽缓解小鼠体力疲劳的作用效果,探究其可能的机制。通过构建急性疲劳与D-半乳糖亚急性衰老2种动物实验模型,考察大豆肽低(0.25 g/kg)、中(0.5 g/kg)、高(2.5 g/kg)剂量下,小鼠负重游泳(WLS)时间、小鼠血清或肝脏中血清尿素氮(BUN)、血乳酸(LD)、糖原、糖原合成酶(GS)、总过氧化物酶(T-SOD)、丙二醛(MDA)、磷酸肌酸激酶(CPK)生化指标的变化。结果表明,2种模型中,各剂量大豆肽均能显著提高小鼠力竭游泳时间,降低BUN含量,然而对MDA含量及CPK活性基本无影响;各剂量大豆肽均能提高小鼠肝脏T-SOD活性,在D-半乳糖亚急性衰老模型中的提高程度小于急性疲劳模型;除D-半乳糖亚急性模型中的低剂量大豆肽对小鼠LD含量的消除无作用外,2种模型其余各剂量大豆肽均可降低LD含量。在急性疲劳模型下,大豆肽能提高小鼠肝糖原贮存量,在D-半乳糖亚急性衰老模型下,大豆肽对其影响不明显,且2组模型中大豆肽对GS活性影响均不显著。结论:在急性疲劳模型下,大豆肽主要通过提高T-SOD活性,减少疲劳产物,提高能源物质积累来达到缓解体力疲劳的作用。比较而言,D-半乳糖亚急性衰老模型与急性模型缓解体力疲劳途径大致相同,而大豆肽对能源物质积累无影响,且缓解疲劳作用效果不强。This Evaluation was aimed to assess the effect of soybean peptide on relieving physical fatigue in mice and explore its mechanism.Two animal experimental models of acute fatigue and D-galactose subacute aging were established.The ICR mice were divided into low-dose(0.25 g/kg),medium-dose(0.5 g/kg)and high-dose(2.5 g/kg)groups of soybean peptide.The load swimming time(WLS),serum urea nitrogen(BUN),blood lactic acid(LD),glycogen,glycogen synthetase(GS),total peroxidase(T-SOD),malondialdehyde(MDA),and creatine phosphate kinase(CPK)in serum and liver of mice were researched.The results showed that in the two models,each dose of soybean peptide could significantly increase exhaustive swimming time and decrease BUN content,but had no effect on MDA content and CPK activity.The activity of T-SOD in the liver of mice was increased by all doses of soybean peptide,and the degree of improvement in the D-galactose subacute aging model was less than that in the acute fatigue model.In the subacute model of D-galactose,low doses of soybean peptides(SP-L)had no effect on the elimination of LD content,and all other doses of soybean peptides in the two models could reduce LD content.Under the acute fatigue model,liver glycogen storage could be increased by soybean peptides but it had not changed significantly in the D-galactose subacute aging model.In conclusion,under the acute fatigue model,soybean peptide can relieve physical fatigue mainly by increasing the activity of T-SOD,reducing fatigue products,and increasing the accumulation of energy substances.The fatigue alleviating pathway of the two models was similar,but the soybean peptide in the D-galactose subacute aging model had no effect on the accumulation of energy substances,and the fatigue alleviating effect was insignificant.

关 键 词：大豆肽 急性疲劳模型 D-半乳糖亚急性衰老模型 缓解体力疲劳 

分 类 号：TS201.4[轻工技术与工程—食品科学]