气相色谱-质谱联用法检测酒醅中氨基甲酸乙酯的方法研究  

Determination of Ethyl Carbamate in Fermented Grains by GC-MS

作　　者：常瑞红[1] 刁亚琴 郭亚飞[1] 谢旭 洪胜 姜勇[1] 徐丽 CHANG Ruihong;DIAO Yaqin;GUO Yafei;XIE Xu;HONG Sheng;JIANG Yong;XU Li(Yanghe Distillery Co.Ltd.,Suqian,Jiangsu 223800,China)

机构地区：[1]江苏洋河酒厂股份有限公司,江苏宿迁223800

出　　处：《酿酒科技》2022年第8期131-134,共4页Liquor-Making Science & Technology

摘　　要：本研究建立了乙醇溶剂萃取结合GC-MS直接进样检测酒醅中EC的测定方法。样品经60%乙醇溶液(V/V)萃取,3.0%(W/V)活性炭净化处理,气相色谱-质谱联用检测酒醅中的EC。结果表明,在浓度为10~800μg/L的范围内线性良好(R2=1),检出限为2.8μg/kg,定量限为9.2μg/kg,加标回收率为88.53%~117.25%,相对标准偏差为1.74%~6.17%。该方法样品处理简便、成本低廉,方法可靠,满足酒醅中EC的检测需要。A method for the determination of ethyl carbamate(EC)in fermented grains was developed by combining ethanol solution extraction with gas chromatography-mass spectrometry(GC-MS)direct injection.The samples were extracted by 60%(v/v)ethanol solution,and purified by 3.0%(w/v)activated carbon.The results showed that,the method had good linear relationship in the concentration range of 10μg/L to 800μg/L(R2=1),the limit of detection was 2.8μg/kg,the limit of quantitation was 9.8μg/kg,the spiked recoveries were 88.53%to 117.25%,and the relative standard deviations were 1.74%to 6.17%.The method is cheap,simple,accurate and reliable,and can meet the requirements for the determination of EC in fermented grains.

关 键 词：酒醅 活性炭:氨基甲酸乙酯 气相色谱-质谱联用仪 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS261.7[轻工技术与工程—食品科学与工程]