分散固相萃取-高效液相色谱法测定饮料和糖果中3种人工合成色素  

Determination of 3 kinds of synthetic pigments in beverages and candies by dispersive solid phase extraction-high performance liquid chromatography

作　　者：许彤 张毅 严恒 谢卫红 XU Tong;ZHANG Yi;YAN Heng;XIE Wei-Hong(College of Biotechnology and Food,Hubei University of Technology,Wuhan 430068,China;Hubei Provincial Institute for Food Supervision and Test,Wuhan 430075,China)

机构地区：[1]湖北工业大学生物工程与食品学院,武汉430068 [2]湖北省食品质量安全监督检验研究院,武汉430075

出　　处：《食品安全质量检测学报》2022年第14期4590-4597,共8页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(20875024)。

摘　　要：目的以聚苯乙烯-聚4-乙烯基吡啶(polystyrene-poly 4-vinylpyridine,PS@P4VP)复合微球为固相萃取材料,分析检测饮料和糖果中柠檬黄、赤藓红和日落黄3种人工合成色素。方法通过吸附pH、吸附时间、等温吸附等条件实验探究了PS@P4VP的吸附性能与最佳固相萃取条件,用高效液相色谱法定量分析了芬达(橙味)汽水和水果糖中3种色素的含量。结果PS@P4VP对柠檬黄和日落黄的最佳吸附pH为3.0,吸附平衡时间分别为20、90 min;对赤藓红的最佳吸附pH为5.0,吸附平衡时间为60 min。PS@P4VP对柠檬黄、赤藓红和日落黄的吸附过程均符合准二级动力学模型和Langmuir等温吸附模型,最大吸附载量分别为47.17、32.47、43.67mg/g。将该材料应用于芬达(橙味)汽水和水果糖中3种色素的检测,加标回收率为89.94%~105.51%,相对标准偏差不超过4.07%(n=6)。结论PS@P4VP对3种色素有较好的吸附性能,且可多次重复使用,将其作为固相萃取材料建立的检测方法准确、可靠,可用于饮料和糖果中柠檬黄等阴离子型人工合成色素的检测。Objective To prepare polystyrene-poly 4-vinylpyridine(PS@P4VP)composite microsphere for the detection and analysis of tartrazine,erythrosine and sunset yellow in carbonated beverages and candies.Methods The adsorption properties of PS@P4VP and the optimum solid phase extraction conditions were explored through experiments on adsorption pH,adsorption time,isothermal adsorption and other conditions,the content of 3 kinds of pigments in Fanta(orange flavor)and fruit candies were quantitatively analyzed by high performance liquid chromatography.Results The optimal adsorption pH of PS@P4VP for tartrazine and sunset yellow was 3.0,and the adsorption equilibrium time was 20 min and 90 min,respectively;the optimal adsorption pH for erythrosine was 5.0and the adsorption equilibrium time was 60 min.The adsorption process of PS@P4VP for tartrazine,erythrosine and sunset yellow followed the pseudo-second order kinetic model and Langmuir isothermal adsorption model,and the maximum adsorption capacities were 47.17,32.47 and 43.67 mg/g,respectively.The material had been applied for the detection of 3 kinds of pigments in Fanta(orange flavor)and fruit candies;the recovery rates of spiked samples were 89.94%~105.51%,and the relative standard deviations were not more than 4.07%(n=6).Conclusion PS@P4VP has good adsorption performance for 3 kinds of pigments,and can be repeatedly used,as a solid phase extraction material,the detection method established by PS@P4VP is accurate and reliable,and can be used for the detection of anionic synthetic pigments such as lemon yellow in beverages and candies.

关 键 词：固相萃取 合成色素 高效液相色谱法 饮料 糖果 

分 类 号：O657.72[理学—分析化学] TS27[理学—化学] TS247[轻工技术与工程—农产品加工及贮藏工程]