男性9号染色体臂间倒位inv(9)(p12q13)与精液质量的关系分析  

Analysis of the relationship between the pericentric inversion inv(9)(p12q13)of male chromosome 9 and semen quality

作　　者：王芬芬 赵丽娟 刘雯 史露露 解磊 杨斯桀 李嘉冬 王鑫 高选 WANG Fenfen;ZHAO Lijuan;LIU Wen;SHI Lulu;XIE Lei;YANG Sijie;LI Jiadong;WANG Xin;GAO Xuan(Department of Clinical Laboratory,Center for Reproductive Medicine,Shandong University/Key Laboratory of Reproductive Endocrinology of Ministry of Education,Shandong University/Shandong Key Laboratory of Reproductive Medicine/Shandong Provincial Clinical Research Center for Reproductive Health/Shandong Technology Innovation Center for Reproductive Health/National Research Center for Assisted Reproductive Technology and Reproductive Genetics,Shandong University,Ji′nan,Shandong 250012,China)

机构地区：[1]山东大学附属生殖医院检验科/生殖内分泌教育部重点实验室(山东大学)/山东省生殖医学重点实验室/山东省生殖健康临床医学研究中心/山东省生殖健康技术创新中心/国家辅助生殖与优生工程技术研究中心,山东济南250012

出　　处：《检验医学与临床》2022年第16期2169-2172,共4页Laboratory Medicine and Clinic

基　　金：山东省医药卫生科技发展计划项目(2017WS107)。

摘　　要：目的探讨9号染色体臂间倒位inv(9)(p12q13)与精液质量的关系。方法选取2018年1月至2019年12月于该院就诊的151例9号染色体臂间倒位inv(9)(p12q13)男性患者作为病例组,选取同期就诊的201例染色体核型正常的男性作为对照组。通过计算机辅助精子分析(CASA)系统检测精液常规参数,同时采用改良巴氏染色法进行精子形态学染色。比较两组精液常规参数及精子形态学指标。结果病例组检出无精子症8例,占5.3%,对照组检出无精子症1例,占0.5%。病例组和对照组精子浓度、精子总数、精液量、非前向运动精子百分比、前向运动精子百分比、不活动精子百分比、精子总活力、畸形精子指数、畸形精子比例、正常形态精子比例及头部、中段、尾部缺陷精子比例差异无统计学意义(P>0.05);对照组精子存活率高于病例组,精子存活率异常比例低于病例组,差异有统计学意义(P<0.05)。结论男性9号染色体臂间倒位inv(9)(p12q13)与精子存活率有一定的关联性,对精液质量存在一定影响。Objective To explore the relationship between the pericentric inversion inv(9)(p12q13)of male chromosome 9 and semen quality.Methods A total of 151 male patients with pericentric inversion inv(9)(p12q13)of chromosome 9 who were treated in this hospital from January 2018 to December 2019 were selected as the case group.A total of 201 males with normal karyotype who visited the clinic during the same period were selected as the control group.The routine parameters of semen were detected by computer-assisted sperm analysis(CASA)system,at the same time,the modified Pap staining method was used to stain the sperm morphology.The semen routine parameters and sperm morphological indexes were compared between the two groups.Results Eight cases of azoospermia were detected in the case group,accounting for 5.3%,and one case of azoospermia was detected in the control group,accounting for 0.5%.There were no significant differences in sperm concentration,total sperm count,semen volume,percentage of non-forward motile sperm,percentage of forward motile sperm,percentage of immotile sperm,total sperm motility,abnormal sperm index,percentage of abnormal sperm,percentage of normal sperm,percentage of sperm with head,middle and tail defects between the case group and the control group(P>0.05).The sperm survival rate in the control group was higher than that in the case group,and the percentage of abnormal sperm survival rate was lower than that in the case group,and the differences were statistically significant(P<0.05).Conclusion The pericentric inversion inv(9)(p12q13)of male chromosome 9 has a certain correlation with sperm survival rate,and has a certain impact on semen quality.

关 键 词：9号染色体 臂间倒位 精液质量 精子存活率 

分 类 号：R394.2[医药卫生—医学遗传学]