柔性玉米醇溶蛋白纳米颗粒负载叶黄素能力的研究  

Study on Xanthophyll Loading Capacity of Flexible Zein Nanoparticles

作　　者：董世荣 马春雪 贺泓睿 DONG Shi-rong;MA Chun-xue;HE Hong-rui(College of Food Engineering,Harbin University,Harbin 150086,China)

机构地区：[1]哈尔滨学院食品工程学院,哈尔滨150086

出　　处：《中国调味品》2022年第9期190-193,198,共5页China Condiment

基　　金：国家自然科学基金(31801485);黑龙江省自然科学基金(LH2020C064);黑龙江省大学生创新创业项目(202110234043,202010234015)。

摘　　要：玉米醇溶蛋白分子刚性较强,利用酸诱导脱酰胺提高玉米醇溶蛋白的分子柔性。采用反溶剂法制备柔性玉米醇溶蛋白纳米颗粒,研究其对叶黄素的负载能力。结果表明,柔性玉米醇溶蛋白可以自组装形成纳米颗粒,柔性玉米醇溶蛋白纳米颗粒对叶黄素具有较好的运载能力。柔性玉米醇溶蛋白纳米颗粒表面或总叶黄素的含量均高于天然玉米醇溶蛋白纳米颗粒。叶黄素添加量为4.8%时,柔性玉米醇溶蛋白纳米颗粒表面和总叶黄素含量分别达到0.527μg/mL和1.411μg/mL。柔性玉米醇溶蛋白纳米颗粒对叶黄素的高负载能力归因于蛋白质分子的无序化,提高了与叶黄素的结合能力。玉米醇溶蛋白二级结构的改变源于脱酰胺改变了蛋白质的氨基酸组成。Zein possesses stronger molecule rigidity.Acids induced deamidation is used to improve the molecular flexibility of zein.Flexible zein nanoparticles are prepared by anti-solvent method to study their loading capacity to xanthophyll.The results show that flexible zein could self-assemble into nanoparticles,and flexible zein nanoparticles have good transport capacity for xanthophyll.The surface or total xanthophyll content of flexible zein nanoparticles is higher than that of natural zein nanoparticles.When the addition amount of xanthophyll is 4.8%,the surface and total xanthophyll content of flexible zein nanoparticles is 0.527,1.411μg/mL respectively.The high loading capacity of flexible zein nanoparticles to xanthophyll is attributed to the disorder of protein molecules,which improves the binding capacity with xanthophyll.The change of secondary structure of zein results from the change of amino acid composition of the protein by deamidation.

关 键 词：玉米醇溶蛋白 脱酰胺反应 叶黄素 二级结构 氨基酸组成 

分 类 号：TS201.21[轻工技术与工程—食品科学]