高效液相色谱法测定茶饮料中的咖啡因含量  

Determination of Caffeine in Tea Drinks by HPLC

作　　者：宁炜 NING Wei(Shanxi Inspection and Testing Center Shanxi Institute of Standard Measurement Technology,Taiyuan 030012,China)

机构地区：[1]山西省检验检测中心(山西省标准计量技术研究院),山西太原030012

出　　处：《食品安全导刊》2022年第22期35-37,共3页China Food Safety Magazine

摘　　要：建立了高效液相色谱法测定茶饮料中咖啡因含量的分析方法。试样经预处理后,过0.45μm微孔水相滤膜,经Promosil C_(18)色谱柱分离,以甲醇和纯水为流动相,等度洗脱,二极管阵列检测器检测。结果表明,咖啡因在0.059~1.960μg/mL与4.91~196.30μg/mL时线性关系良好,线性系数均大于0.999,加标回收率为96.43%~103.31%,方法的重复性RSD为1.56%,检出限为0.059 mg/kg,定量限为0.2 mg/kg。该方法前处理简便,定性定量准确,可用于茶饮料中咖啡因含量的批量快速检测。The method for the determination of caffeine in tea beverage by high performance liquid chromatography was established.After pretreatment,the sample passes 0.45μm microporous aqueous phase filter membrane,separated by Promosil C_(18) chromatographic column,eluted with pure water and methanol as mobile phase,and detected by diode array detector.The results showed that caffeine ranged from 0.059~1.960μg/mL and 4.91~196.30μg/mL,the linear relationship within was good,the linear coefficients were greater than 0.999,the recovery was 96.43%~103.31%,the repeatability RSD of the method was 1.56%,the detection limit was 0.059 mg/kg,and the quantitative limit was 0.2 mg/kg.The method is simple in pretreatment,accurate in qualitative and quantitative analysis,and can be used for rapid batch determination of caffeine in tea drinks.

关 键 词：咖啡因 茶饮料 高效液相色谱法 

分 类 号：TS275.2[轻工技术与工程—农产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]