正交试验法优化巨大口蘑子实体中粗多糖提取工艺的研究  

Optimization of Extraction Process of Crude Polysaccharides from Macrocybe giganteum by Orthogonal Experimental Design

作　　者：陈梅梅 周于轩 CHEN Meimei;ZHOU Yuxuan(Guangzhou College of Technology and Business,Guangzhou 510000,China)

机构地区：[1]广州工商学院,广东广州510000

出　　处：《食品安全导刊》2022年第22期132-135,共4页China Food Safety Magazine

摘　　要：本研究利用正交试验法优化巨大口蘑粗多糖水提法工艺,以提高其提取率。单因素试验选取了提取时间、提取温度、料液比和滤液与无水乙醇的体积比这4个因素,以提取率作为评价标准。在此基础上设计4因素3水平的正交试验,对其提取工艺进行优化。结果表明,影响巨大口蘑子实体中粗多糖提取率的主要因素为料液比,其次是提取时间和滤液与无水乙醇的体积比,而提取温度对其提取率的影响最低;最佳提取工艺为滤液与无水乙醇体积比为1∶2、提取温度为70℃、料液比为1∶15、提取时间为1.0 h,在此条件下巨大口蘑的多糖提取率可达8.42%。In this study,orthogonal experimental design was used to optimize the process of crude polysaccharides from Macrocybe giganteum with the method of water extraction.The one-factor experimental design was adopted with the temperature,time,solid-liquid ratio and the ratio of the filter liquor to the absolute ethanol.The yield rate of the crude polysaccharides was the evaluation standard.Then an orthogonal test of four factors at three different levels were designed and used.The results indicated that the most important factor that infect the yield rate was the temperature and the best extraction process were:the ratio of the flter liquor to the absolute ethanol was 1∶2,the temperature was 70℃,the solid-liquid ratio was 1∶15,and the time was 1.0 hour.With the best extraction process the yield rate was 8.42%.

关 键 词：巨大口蘑 多糖 正交试验 水提法 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]