紫苏籽粕抗氧化肽的纯化、鉴定及降血脂功效研究  

Effects of Perilla Seed Meal Antioxidant Peptides on Purification,Identification and Lipid-Lowering

作　　者：白宝清[1,2] 贾槐旺 张锦华 李兰[1,2] 范三红 Bai Baoqing;Jia Huaiwang;Zhang Jinhua;Li Lan;Fan Sanhong(College of Life Science,Shanxi University,Taiyuan 030006;Shanxi Key Laboratory for Research and Development of Regional Plants,Shanxi University,Taiyuan 030006)

机构地区：[1]山西大学生命科学学院,太原030006 [2]特色植物资源研究与利用山西重点实验室,山西大学,太原030006

出　　处：《中国粮油学报》2022年第7期92-101,共10页Journal of the Chinese Cereals and Oils Association

基　　金：山西省重点研发计划重点项目(201703D211019,201703D211012-1),山西省专利推广实施资助专项(20171002),山西省“1331工程”山西特色生物资源与健康产业协同创新中心(2017),2021年度山西省产教融合研究生联合培养示范基地(2021JD04),2020年度研究生教育创新计划项目(2020SY022),山西大学杏花村学院(山西酿造产业研究院(筹))开放基金(XCSXU-KF-202001、XCSXU-KF-202008,XCSXU-KF-202012)。

摘　　要：使用碱性蛋白酶对紫苏籽粕蛋白酶解,酶解物依次通过超滤和柱层析进行分离纯化,纯化肽经LC-MS-MS鉴定并通过动物实验对其进行降血脂及抗氧化功能性研究。结果表明,蛋白酶解物经超滤分离后,其分子质量小于3 ku的组分具有较高的抗氧化活性、该组分经DEAE-32离子交换色谱分离后,得到D1、D2、D3共3个组分,其中D3的抗氧化活性最好,D3经Sephadex G-25凝胶层析分离纯化后,富集得到G1、G2、G3三个组分,组分G2抗氧化活性最高。抗氧化活性肽G2对1,1-二苯基-2-三硝基苯肼(DPPH)、2,2′-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)、超氧阴离子(O_(2)^(-))·、羟基(·OH)自由基清除率及还原力依次为70.00%、47.09%、51.17%、70.04%、0.742;通过LC-MS-MS鉴定,抗氧化活性肽G2组分由7个氨基酸组成,分子质量为790.3755 u,其氨基酸序列为Ala-Ala-Asp-Cys-Arg-Ala-Lys。经低、中、高剂量组G2灌胃处理后的小鼠相比高脂模型组,小鼠体内的MDA(丙二醛)含量显著或极显著下降(P<0.05或P<0.01);小鼠的GSH-Px(谷胱甘肽过氧化物酶)、SOD(超氧化物歧化酶)和CAT(过氧化氢酶)活力显著或极显著升高(P<0.05或P<0.01);小鼠体内的TC(总胆固醇)、TG(甘油三酯)和LDL-C(低密度脂蛋白胆固醇)含量显著或极显著降低(P<0.05或P<0.01),HDL-C(高密度脂蛋白胆固醇)含量显著或极显著升高(P<0.05或P<0.01)。Alkaline proteases were usedfor enzymolysis of perila seed meal,and the zymolytes were separated and purified byultrafiltration and column chromatography.The purfied peptide was idenfified by,and the research on liqid lowering and antioxidantfunction were conducted by animal experiment.The results indicatedthat after the protease wasseparated by ultrafiltration,the component having a molecular weight of less than 3 ku hada high antioxidant activity,and three components of D1,D2,D3 were obtained by DEAE-32 ion exchange chromatography.Wherein,the antioxidant activity of D3 was the highested.After D2 was separated and purified by G-25 gel chromatography,three components of G1,G2,G3 was obtained by enrichment,and the component G2 has the highest antioxidant activity.The scavenging rates and reducing power of antioxidant peptide G2 to 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH),2,2′-diazo-bis-3-ethylbenzothiazoline-6-sulfonic acid(ABTS),superoxide anion(O_(2)^(-))and hydroxyl(·OH)radical were 70.00%,47.09%,51.17%and 70.04%,respectively,and the reducing power of G1 was 0.742.The antioxidant active peptide G2 components were composed of 7 amino acids by LC-MS-MS,and the molecular mass was 790.3755 u,and the amino acid sequence was Ala-Ala-ASP-CYS-ARG-ALA-ALAS.Compared with the high-fat model group,the content of MDA in mice treated by G2 gavage in low,middle and high dose groups decreased significantly or extremely significantly(P<0.05 or P<0.01);GSH-Px,SOD and CAT viability in mise was significanly or extremely significantly incaresed(P<0.05 or P<0.01);TC,Tg and LDL-C in mice significantly orextremely significantly decreased(P<0.05 or P<0.01),HDL-The C content was significantly orextremely significantly increased(P<0.05 or P<0.01).

关 键 词：紫苏籽饼粕 分离纯化 抗氧化活性肽 降血脂 

分 类 号：TS201.1[轻工技术与工程—食品科学]