pH驱动制备纳他霉素纳米乳液及其结构表征  

Preparation and structure characterization of natamycin nanoemulsion based on pH driven method

作　　者：牛超杰 汪慧 常超 NIU Chao-jie;WANG Hui;CHANG Chao(School of Food Science and engineering,Wuhan polytechnic University,Wuhan 430070,China)

机构地区：[1]武汉轻工大学食品科学与工程学院,武汉430023

出　　处：《武汉轻工大学学报》2022年第4期16-23,共8页Journal of Wuhan Polytechnic University

基　　金：大宗粮油精深加工教育部重点实验室开放课题(编号:2020JYBQGDKFB02).

摘　　要：利用脱脂奶粉、纳他霉素和Na-CMC的pH驱动制备纳他霉素纳米乳液,制备高包封率且稳定性好的纳米乳液,同时通过AFM对纳米乳液的微观形貌进行表征并考察了纳米乳液的贮藏稳定性,借助FTIR、XRD衍射分析对纳他霉素纳米乳液的结构进行表征。结果发现,纳他霉素纳米乳液的最佳制备条件为脱脂奶粉浓度为10 mg/mL、纳他霉素浓度为12 mg/mL和Na-CMC浓度为4 mg/mL,三者体积比为1∶1∶1。在此条件下,纳米粒粒径约为238.46 nm,Zeta电位约为-42.8 mV,包封率约为92.14%,在4℃下贮藏30 d性能保持稳定。AFM结果显示纳米乳液的分散性相对良好,FTIR结果显示纳他霉素纳米乳液没有出现新的特征峰,分子间作用力和氢键作用都有所减弱。XRD衍射分析表明纳米乳液中被包埋的纳他霉素晶型改变。本研究结果可为拓展纳他霉素的实际应用范围提供理论依据。Natamycin nanoemulsion were fabricated based on the pH driven method of skimmed milk powder,natamycin and Na-CMC and the process parameters to form stable nanoparticles with high encapsulation rate Meanwhile,the morphological attributes of micro emulsion were characterized by AFM and the storage stability of nanoemulsion was studied.Furthermore,the structure of the natamycin nanoemulsion was researched by FTIR and XRD diffraction analysis.The results showed that the formation of stable natamycin nanoemulsion with high encapsulation rate was achieved under the following conditions:the concentration of skimmed milk powder was 10 mg/mL,the concentration of natamycin was 12 mg/mL,the concentration of Na-CMC was 4 mg/mL,and the volume ratio of the three was 1∶1∶1.Under these process conditions,the particle size of nanoparticles was about 238.46 nm,the zeta potential was about-42.8 mV,the encapsulation rate was about 94.12%.Moreover,the natamycin nanoemulsion were remained stable after storage of one month at 4℃.AFM results showed that the dispersion of natamycin nanoemulsion is relatively good.According to the results of FTIR,no new characteristic peaks in the natamycin nanoemulsion,but the intermolecular forces and hydrogen bonding were all weakened.XRD diffraction analysis indicated that the crystal form of the embedded natamycin in the nanoemulsion changed.The results of this study can provide a theoretical basis for expanding the practical application scope of natamycin.

关 键 词：脱脂奶粉 纳他霉素 羧甲基纤维素钠 纳米乳液 

分 类 号：TS207.4[轻工技术与工程—食品科学]