基于培养基优化提高过表达MET14基因的重组库德毕赤酵母M48脱镉能力的研究  

Study on Improving the Cadmium Removal Ability of Recombinant Pichia kudriavzevii M48 Overexpressed MET14 Gene Based on Medium Optimization

作　　者：徐婉莹 戚晓雪 徐莹[1] 汪东风[1] XU Wanying;QI Xiaoxue;XU Ying;WANG Dongfeng(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品工业科技》2022年第19期288-297,共10页Science and Technology of Food Industry

基　　金：国家自然科学基金(31571927)。

摘　　要：对一株具有高镉抗性和镉脱除率的过表达MET14基因的重组库德毕赤酵母(Pichia kudriavzevii) M48进行培养基优化研究,以其脱镉率和生物量的综合值为评价指标,通过单因素实验、25-1部分析因设计和中心组合设计优化培养基成分,为后期将重组菌株M48做成脱镉制剂提供研究基础。结果表明,最优培养基成分为:葡萄糖23.42 g/L,酵母浸粉27.72 g/L,磷酸二氢钾2.04 g/L。在此条件下得到的M48脱镉率为79.63%,生物量为9.95 g/L,分别是未优化前的2.70倍和1.16倍。本研究通过优化重组菌株M48的培养基成分,有效提高了其脱镉率和生物量。In this study, the culture medium of a recombinant Pichia kudriavzevii M48 overexpressed MET14 gene with high cadmium resistance and cadmium removal rate was optimized. Taking the comprehensive value of cadmium removal rate and biomass as the evaluation index, the medium composition was optimized through single factor test, 25-1 fractional factorial design and central composite design, so as to provide a research basis for making the recombinant strain M48 into cadmium removal preparation in the later stage. The results showed that the optimal medium was 23.42 g/L glucose,27.72 g/L yeast extract and 2.04 g/L of potassium dihydrogen phosphate. Under these conditions, the cadmium removal rate of M48 was 79.63% and the biomass was 9.95 g/L, which were 2.70 and 1.16 times higher than that before optimization,respectively. Therefore, the cadmium removal rate and biomass of recombinant strain M48 were effectively increased by optimizing the medium composition.

关 键 词：库德毕赤酵母 重组菌株 培养基优化 脱镉率 生物量 

分 类 号：TS201.6[轻工技术与工程—食品科学]