Artificial nerve graft constructed by coculture of activated Schwann cells and human hair keratin for repair of peripheral nerve defects  

作　　者：Han-Jun Qin Hang Li Jun-Ze Chen Kai-Rui Zhang Xing-Qi Zhao Jian-Qiang Qin Bin Yu Jun Yang 

机构地区：[1]Department of Orthopedics,Nanfang Hospital,Southern Medical University,Guangdong Provincial Key Laboratory of Bone and Cartilage Regenerative Medicine,Nanfang Hospital,Southern Medical University,Guangzhou,Guangdong Province,China [2]Hebei Medical University,Shijiazhuang,Hebei Province,China [3]Department of Orthopedics,Baiyun Branch of Southern Hospital,Guangzhou,Guangdong Province,China [4]Department of Anatomy,School of Basic Medical Sciences,Southern Medical University,Guangzhou,Guangdong Province,China [5]Department of Orthopedics,The 74th Group Military Hospital of PLA,Guangzhou,Guangdong Province,China

出　　处：《Neural Regeneration Research》2023年第5期1118-1123,共6页中国神经再生研究（英文版）

基　　金：supported by Military Medical Science&Technology Youth Training Program,No. 19QNP005;President Foundation of Nanfang Hospital,Southern Medical University,No. 2020B028 (both to JY)。

摘　　要：Studies have shown that human hair keratin(HHK) has no antigenicity and excellent mechanical properties. Schwann cells, as unique glial cells in the peripheral nervous system, can be induced by interleukin-1β to secrete nerve growth factor, which promotes neural regeneration. Therefore, HHK with Schwann cells may be a more effective approach to repair nerve defects than HHK without Schwann cells. In this study, we established an artificial nerve graft by loading an HHK skeleton with activated Schwann cells. We found that the longitudinal HHK microfilament structure provided adhesion medium, space and direction for Schwann cells, and promoted Schwann cell growth and nerve fiber regeneration. In addition, interleukin-1β not only activates Schwann cells, but also strengthens their activity and increases the expression of nerve growth factors. Activated Schwann cells activate macrophages, and activated macrophages secrete interleukin-1β, which maintains the activity of Schwann cells. Thus, a beneficial cycle forms and promotes nerve repair. Furthermore, our studies have found that the newly constructed artificial nerve graft promotes the improvements in nerve conduction function and motor function in rats with sciatic nerve injury, and increases the expression of nerve injury repair factors fibroblast growth factor 2 and human transforming growth factor B receptor 2. These findings suggest that this artificial nerve graft effectively repairs peripheral nerve injury.

关 键 词：artificial nerve graft bioactive human hair keratin INTERLEUKIN-1Β MACROPHAGES nerve graft nerve growth factor nerve repair peripheral nervous injury Schwann cells 

分 类 号：R741[医药卫生—神经病学与精神病学]