水产品中5种磷脂含量的HPLC-ELSD检测分析  

Determination and Analysis for Contents of Five Phospholipids in Aquatic Products by HPLC-ELSD Method

作　　者：赵新楠 彭吉星[1,2] 吴海燕 郑关超[1,2] 郭萌萌 文艺晓 王联珠[1,2] 谭志军 ZHAO Xinnan;PENG Jixing;Wu Haiyan;ZHENG Guanchao;GUO Mengmeng;WEN Yixiao;WANG Lianzhu;TAN Zhijun(Key Laboratory of Testing and Evaluation for Aquatic Product Quality and Safety,Ministry of Agriculture and Rural Affairs,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;Laboratory of Aquatic Product Quality and Safety Risk Assessment,Ministry of Agriculture and Rural Affairs,Qingdao 266071,China)

机构地区：[1]农业农村部水产品质量安全检测与评价重点实验室,中国水产科学研究院黄海水产研究所,青岛266071 [2]农业农村部水产品质量安全风险评估实验室,青岛266071

出　　处：《中国渔业质量与标准》2022年第4期10-17,共8页Chinese Fishery Quality and Standards

基　　金：中国水产科学研究院黄海水产研究所基本科研业务费项目(20603022022009);中国水产科学研究院基本科研业务费项目(2020TD48);国家重点研发计划(2018YFD0901004)。

摘　　要：本研究建立了水产品中5种磷脂的高效液相色谱-蒸发光散射检测器(HPLC-ELSD)方法并测定了几种水产品及水产品性腺组织中5种磷脂的含量。采用氯仿-甲醇(2∶1,V/V)进行磷脂的提取,氨基固相萃取柱净化富集,HPLC-ELSD对牡蛎(Ostrea gigas tnunb)、虹鳟(Oncorhynchus mykiss)、刺参(Apostichopus japonicas)、磷虾(Euphausia)以及海胆(Echinoidea)黄、大闸蟹(Eriocheir sinensis)蟹黄、鲟鱼(Acipenser sinensis)子酱、刺参性腺中的磷脂组成和含量进行分析,该方法加标回收率为77%~102%,相对标准偏差为0.75%~9.78%,具有较好的回收率和良好的精密度。结果显示,上述样品中含量最高的磷脂均为磷脂酰胆碱,其中磷虾粉中磷脂酰胆碱含量最高为29.71~35.30 mg/g。磷脂酰乙醇胺除鲟鱼子酱样品外均有检出,其中刺参和牡蛎冻干粉样品中磷脂酰乙醇胺含量较高,含量范围分别为2.90~3.69 mg/g和2.49~4.40 mg/g,磷脂酰肌醇、溶血磷脂酰胆碱和鞘磷脂3种磷脂仅有少数品种有检出,且含量较低。综上,HPLC-ELSD法可以实现对鱼、贝、虾等水产品中5种磷脂组分的快速准确分析及定量,为水产品营养品质评价提供参考。In this study,a high performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD)method for 5 kinds of phospholipids in aquatic products was established,and then their contents in several aquatic products and gonadal tissues were determined.The mixture solution of chloroform and methanol(2∶1,v/v)was used to extract phospholipids,and amino solid-phase extraction column was applied to purify and enrich.The composition and content of phospholipids in Ostrea gigas tnunb,Oncorhynchus mykiss,Apostichopus japonicas,Euphausia,Echinoidea,crab spawn of Eriocheir sinensis and caviar of Acipenser sinensis were analyzed by HPLC-ELSD.The recoveries of this method were in ranges of 77%-102%with relative standard deviation(RSD)of 0.75%-9.78%,indicating that the method had good recoveries and satisfactory precision.The highest content of phospholipid in these aquatic products was phosphatidylcholines,among which the highest content of phosphatidylcholines in krill meal ranged from 29.71 mg/g to 35.30 mg/g.Phosphatidylethanolamine was detected in all above samples except sturgeon caviar,and the contents of phosphatidylethanolamine in the freeze-dried powder samples of stichopus japonicus and oyster were higher with content ranges of 2.90-3.69 mg/g and 2.49-4.40 mg/g.Phosphatidylinositol,lysophosphatidylcholine and sphingomyelin were only detected in a few varieties with low contents.In conclusion,the HPLC-ELSD method could realize the rapid and accurate analysis and quantification for five kinds of phospholipid components in fish,shellfish,shrimp and other aquatic products to provide a reference for the nutritional quality evaluation of aquatic products.

关 键 词：水产品 磷脂 HPLC-ELSD 检测方法 定量分析 营养品质 

分 类 号：S986.1[农业科学—捕捞与储运]