凯氏定氮全定量、半定量法以及杜马斯燃烧法测定米糠粗蛋白优劣势比较  

Application Exploration of Rapid Determination Content Cadmium in Rice by Portable X Fluorescence Analyzer

作　　者：李小丹 朱丽琼[2] 曾彩虹[2] 刘鑫 吴家乾 李丹青 潘传荣[2] LI Xiaodan;ZHU Liqiong;ZENG Caihong;LIU Xin;WU Jiaqian;LI Danqing;PAN Chuanrong(Guangdong Zhanjiang National Grain Quality Monitoring Station,524045 Zhanjiang Guangdong,China;Guangdong Institute for Cereal Science Research,510050 Guangzhou,China)

机构地区：[1]广东湛江国家粮食质量监测站,广东湛江524045 [2]广东省粮食科学研究所有限公司,广州510050

出　　处：《粮食加工》2022年第5期122-126,共5页Grain Processing

基　　金：广东省现代农业产业共性关键技术研发创新团队建设项目(项目编号2022KJ117)。

摘　　要：通过对凯氏定氮法的全定量和半定量法及杜马斯燃烧法测定蛋白质实验的科学比较,分析三种方法的优劣势。作为蛋白质测定的国家标准方法,三种方法检测结果并没有显着性差异,所以测定米糠中粗蛋白含量时采用三种方法均是优良可行的。凯氏定氮法所需设备价格低、成本低廉、已经有着广泛的应用,但是操作时间长,有一定的危险性,产生有毒有害气体,危害人体健康,结果受人为影响;杜马斯燃烧法操作过程简便快捷,时间只需几分钟,不需人看管,不产生有毒有害物质,但是仪器昂贵,检测成本高。The advantages and disadvantages of the three methods are analyzed by the scientific comparison of the full and semi-quantitative method and the Dumas combustion method.As a national standard method for pro-tein determination,the detection results have no significant difference between the three methods,so the three medium medium method is excellent and feasible to determine the crude protein content in rice bran.The nitro-gen method has low price,low cost,and is widely used,but has long operation time,causing toxic and harmful gases,endangering human health,only a few minutes,

关 键 词：蛋白质沉淀 杜马斯燃烧法 全定量凯氏定氮法 半定量凯氏定氮法 

分 类 号：TS210.7[轻工技术与工程—粮食、油脂及植物蛋白工程]