类胡萝卜素对萝卜芽苗菜生长及抗氧化能力的实验研究  

Experimental study of carotenoids on growth and antioxidant capacity of radish sprouts

作　　者：李明慧 姚晓芹[1] 韩超 董丽君[1] 王亚光[1] 楚建周[1] LI Minghui;YAO Xiaoqin;HAN Chao;DONG Lijun;WANG Yaguang;CHU Jianzhou(School of Life Science,Hebei University,Baoding 071002,China;School of Life Science and Engineering,Handan University,Handan 056005,China)

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]邯郸学院生命科学与工程学院,河北邯郸056005

出　　处：《实验技术与管理》2022年第9期37-41,53,共6页Experimental Technology and Management

基　　金：国家自然科学基金资助项目(32071501;31300321);邯郸市科学技术研究与发展计划(1727201061-1)。

摘　　要：用β-胡萝卜素和玉米黄质对萝卜芽苗菜进行处理,得出了不同类胡萝卜素种类和浓度对萝卜芽苗菜下胚轴长度和抗氧化能力的影响。结果表明:与对照相比,β-胡萝卜素和玉米黄质处理均能促进芽苗菜下胚轴长度的增长,降低芽苗菜贮藏后丙二醛(MDA)含量和超氧阴离子(O·~–)生成速率,并且适宜浓度可有效提高芽苗菜贮藏后的抗氧化酶(POD、SOD)活性以及DPPH自由基清除能力。在β-胡萝卜素和玉米黄质处理中,5 mg/L的β-胡萝卜素或20mg/L的玉米黄质处理下萝卜芽苗菜的下胚轴最长;和对照组相比,贮藏后萝卜芽苗菜中O_(2)·^(–)生成速率和MDA含量在5~10 mg/L的β-胡萝卜素和10~20 mg/L的玉米黄质处理下较低;40 mg/L的β-胡萝卜素和20~40mg/L的玉米黄质处理下的萝卜芽苗菜在贮藏后具有较高的POD、SOD活性以及DPPH自由基清除能力。综合分析表明,与β-胡萝卜素处理相比,玉米黄质处理可以使萝卜芽苗菜具有较低的MDA含量和较高的POD活性,更有利于维持芽苗菜的贮藏品质,延缓腐烂的发生。The effects of different kinds and concentrations of carotenoids on hypocotyl length and antioxidant capacity of radish sprouts are studied by treating radish sprouts with β-carotene and zeaxanthin. The results show that β-carotene and zeaxanthin treatments can improve the growth of hypocotyl length, reduce malondialdehyde(MDA) content and the rate of superoxide anion(O_(2)·^(-)) production in stored sprouts compared with the control, and effectively improve the activities of antioxidant enzymes(POD, SOD) and DPPH radical scavenging ability in sprouts after storage at appropriate concentration. The hypocotyl length of radish sprouts treated with 5 mg/L β-carotene and 20 mg/L zeaxanthin is the longest. The rate of O·production and MDA content in radish sprouts treated with 5~10 mg/L β-carotene and 10~20 mg/L zeaxanthin are lower than that of the control after storage.Radish sprouts treated with 40 mg/L β-carotene and 20~40 mg/L zeaxanthin has higher POD, SOD activities and DPPH radical scavenging ability after storage. Comprehensive analysis shows that zeaxanthin treatment can make radish sprouts have lower MDA content and higher POD activity compared with β-carotene treatment, which is more beneficial to maintain the storage quality of sprouts and to delay the occurrence of rot.

关 键 词：萝卜芽苗菜 Β-胡萝卜素 玉米黄质 抗氧化能力 

分 类 号：S636[农业科学—蔬菜学] S131.2[农业科学—园艺学]