Cytological evaluation by Caco-2 and KU812 of non-allergenic peptides from simulated digestion of infant formula in vitro  

作　　者：Zihao Xu Hao Bai Xin Ma Yong Wu Zhihua Wu Anshu Yang Weixiang Mao Xin Li Hongbing Chen 

机构地区：[1]State Key Laboratory Food Science and Technology,Nanchang University,Nanchang 330047,China [2]School of food science and Technology,Nanchang University,Nanchang 330047,China [3]Sino-German Joint Research Institute,Nanchang University,Nanchang 330047,China [4]Jiangxi Province Key Laboratory of Food Allergy,Nanchang University,Nanchang 330047,China [5]Jiangxi Institute of Quality and Standardization,Nanchang 330046,China

出　　处：《Food Science and Human Wellness》2023年第3期817-824,共8页食品科学与人类健康（英文）

基　　金：supported by National Key R&D Program of China(2018YFC1604205);the National Natural Science Foundation of China(31760431);State Key Laboratory of Food Science and Technology,Nanchang University(SKLF-ZZA-201912)。

摘　　要：Milk allergy is a common allergic reaction found in infants and young children,most of them appear tolerance after growing up.In this study,infant formula was digested by simulated in vitro digestion method.The potential non-allergenic peptides were further screened from undigested products by exclusion of the known epitopes fromβ-lactoglobulin(BLG)andα-lactalbumin(ALA).These potential non-allergenic peptides were synthesized and their transferability were determined by Caco-2 cell monolayer model.Finally,the potential allergenicity were evaluated by KU812 cell degranulation model.The results showed that 7 peptides were screened as non-allergenic sequences,among which were 3 from ALA and 4 from BLG.The Caco-2 cell model showed that all the synthetic peptides were absorbed and transported well.However,only peptide BLG_(107-118)showed potential allegencity by KU812 model.In conclusion,6 peptides,including ALA_(29-51),ALA_(80-90),ALA_(94-103),BLG_(1-20),BLG_(24-50),and BLG_(123-139)were evaluated as hypoallergenic peptides,which could be used for candidates of peptides inducing immune tolerance for persons with milk allergy.

关 键 词：Milk allergy EPITOPES PEPTIDES Hypoallergenic Immune tolerance 

分 类 号：TS20[轻工技术与工程—食品科学]