Au@Ag-labeled SERS lateral flow assay for highly sensitive detection of allergens in milk  

作　　者：Jing Li Jia Xu Yi Pan Yongzhi Zhu Yuanfeng Wang Shouhui Chen Xinlin Wei 

机构地区：[1]Department of Food Science and Technology,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China [2]Institute of Food Engineering,College of Life Science,Shanghai Normal University,Shanghai 200234,China

出　　处：《Food Science and Human Wellness》2023年第3期912-919,共8页食品科学与人类健康（英文）

基　　金：supported by the Science and Technology Innovation Action Plan of Shanghai,China(17391901302);the National Key R&D Program of China(2018YFC1604401)。

摘　　要：Casein andα-lactalbumin(α-LA)are the main allergens in cow's milk,which can affect the skin,respiratory system,and gastrointestinal tract,even cause anaphylactic shock.Developing a rapid and sensitive detection method of casein andα-LA is still pursued.Herein,a surface-enhanced Raman scattering based lateral flow assay(SERS-LFA)method for rapid and highly sensitive detection of milk allergens in food was established for effectively preventing allergic symptoms.Gold@silver nanoparticles(Au@Ag NPs)were synthesized as SERS active substrate to prepare the antibody-modified SERS Probe and SERS-LFA strips toward casein andα-LA were assembled according to the sandwich mode.The detection results were calculated according to colorimetric and Raman signal.The introduction of SRES signal significantly increased the sensitivity of detection with the limit of detection(LOD)of 0.19 ng/mL and 1.74 pg/mL,and exhibited an excellent linear relationship within the range of 0.55-791.50 ng/mL and 0.1 pg/mL-100.0 ng/mL for casein andα-LA,respectively.Furthermore,SERS-LFA strips was highly specific with the recovery rates for corresponding80.36%-105.12%and 85.73%-118.22%for casein andα-LA,respectively.Therefore,the SERS-LFA could be in great potential to develop a unique allergen detection method.

关 键 词：CASEIN Α-LACTALBUMIN Au@AgNPs SERS-LFA 

分 类 号：TS201.6[轻工技术与工程—食品科学]