高产虾青素红法夫酵母的代谢工程育种研究进展  

Research Progress in Metabolic Engineering Breeding of High-Yield Astaxanthin-Producing Xanthophyllomyces dendrorhous

作　　者：王军[1] 张晴龙 李曦月 倪孟祥[1] WANG Jun;ZHANG Qinglong;LI Xiyue;NI Mengxiang(China Pharmaceutical University,Nanjing 210000,China)

机构地区：[1]中国药科大学,江苏南京210000

出　　处：《化学与生物工程》2022年第10期1-5,共5页Chemistry & Bioengineering

基　　金：国家重点基础研究发展计划项目(2019YFA090404)。

摘　　要：虾青素具有超强的抗氧化特性,还能够抗肿瘤、提高机体免疫力、抗衰老等,在保健食品行业具有广阔的发展前景。红法夫酵母是目前报道的自然界中唯一合成虾青素的酵母类菌株,在分子水平对红法夫酵母进行基因改造以选育高产虾青素菌株极具应用潜力。在分析虾青素结构及代谢途径的基础上,介绍了红法夫酵母中虾青素的合成限速酶,并对高产虾青素红法夫酵母的代谢工程育种方法的研究进展进行了综述,如过表达虾青素内源性基因、异源表达外源性基因、敲除旁支基因等。Astaxanthin has the characteristics of strong antioxidant property,anti-tumor,enhancing body immunity,anti-aging and so on,which has broad development prospects in the health food industry.Xanthophyllomyces dendrorhous is currently the only yeast strain that can be adopted to synthesize astaxanthin in nature,thus the genetic modification of Xanthophyllomyces dendrorhous at a molecular level to breed high-yield astaxanthin-producing strain has great application potential.In this paper,on the basis of the analyses of the structure and metabolic pathway of astaxanthin,we introduce the rate-limiting enzyme for the biosynthesis of astaxanthin in Xanthophyllomyces dendrorhous,and review the research progress in metabolic engineering breeding methods of high-yield astaxanthin-producing Xanthophyllomyces dendrorhous,such as overexpression of astaxanthin endogenous gene,heterologous expression of exogenous gene,and knockout of collateral gene,etc.

关 键 词：虾青素 红法夫酵母 限速酶 代谢工程 育种 

分 类 号：TQ929[轻工技术与工程—发酵工程]