基于糖蜜原料的γ-聚谷氨酸发酵培养基优化  

Optimization ofγ-Polyglutamic Acid Fermentation Medium Based on Molasses

作　　者：李祥松 王慧超 赵廷彬 张琳 孙银华 李振海 徐雪天 乔长晟[1,2,3,4] 郑保国 LI Xiang-song;WANG Hui-chao;ZHAO Ting-bin;ZHANG Lin;SUN Yin-hua;LI Zhen-hai;XU Xue-tian;QIAO Chang-sheng;ZHENG Bao-guo(College of Biotechnology,Tianjin University of Science&Technology,Tianjin 300457,China;Tianjin Huizhi Baichuan Biological Engineering Co.,Ltd.,Tianjin 300457,China;Tianjin Peiyang Biotrans Biotech Co.,Ltd.,Tianjin 300457,China;Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control,Tianjin 300457,China;Agricultural Biotechnology Research Center,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002,Ningxia,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]天津慧智百川生物工程有限公司,天津300457 [3]天津北洋百川生物技术有限公司,天津300457 [4]天津市微生物代谢与发酵过程控制技术工程中心,天津300457 [5]宁夏农林科学院农业生物技术研究中心,宁夏银川750002

出　　处：《食品研究与开发》2022年第20期186-193,共8页Food Research and Development

基　　金：宁夏回族自治区重点研发计划(2021BEG03011);四川省科技计划重点研发项目(2021YFN0135)。

摘　　要：γ-聚谷氨酸(γ-polyglutamic acid,γ-PGA)是一种新型绿色高分子材料,被广泛应用于农业生产、食品、医药等众多领域。目前γ-PGA生产成本高,产量低等问题较为突出。为降低生产成本,该文以廉价甘蔗糖蜜作为碳源。利用单因素与响应面法优化发酵培养基。结果显示,最佳培养基组成为糖蜜可溶性固形物浓度8.68%、酵母膏浓度4.23 g/L、FeSO_(4)·7H_(2)O浓度0.78 g/L,味精浓度80 g/L,γ-PGA产量为(67.88±0.41)g/L,与预测值67.17 g/L非常接近,相较于优化前γ-PGA产量提高了1.19倍,为工业化生产奠定基础。γ-Polyglutamic acid(γ-PGA)is a novel eco-friendly polymer.It has been widely used in agriculture,food,medicine,and many other industries.To date,the major problem withγ-PGA is its high production cost with low yield.To reduce the production cost,cane molasses(a low cost product)was selected as the carbon source for fermentation,and single factor test and response surface methodology were used to optimize the fermentation medium.The results showed that the optimal medium composition was molasses with 8.68%soluble solid,4.23 g/L yeast extract,0.78 g/L FeSO_(4)·7H_(2)O and 80 g/L monosodium glutamate.Under the above conditions,the yield ofγ-PGA reached(67.88±0.41)g/L,which was similar to the predicted value of 67.17 g/L.The yield increased by about 1.19 times compared with the control.This result could lay the foundation for industrial production.

关 键 词：培养基组成 Γ-聚谷氨酸 响应面优化 甘蔗糖蜜 地衣芽孢杆菌 

分 类 号：TQ920.1[轻工技术与工程—发酵工程] TQ317