马氏珠母贝闭壳肌活性肽制备及其辅助降血糖功效评价  

Preparation of active peptide from Pinctada martensii adductor muscle and evaluation of its auxiliary hypoglycemic effect

作　　者：廖津 林海生 伍彬 秦小明 曹文红 高加龙 郑惠娜 章超桦 谭绮晴 LIAO Jin;LIN Haisheng;WU Bin;QIN Xiaoming;CAO Wenhong;GAO Jialong;ZHENG Huina;ZHANG Chaohua;TAN Qiqing(College of Food Science and Technology,Guangdong Ocean University/National Research and Development Branch Center for Shellfish Processing(Zhanjiang)/Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety/Guangdong Province Engineering Laboratory for Marine Biological Products/Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution,Zhanjiang 524088,China;Department of Food Science and Engineering,Jinan University,Guangzhou 510632,China;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian 116034,China)

机构地区：[1]广东海洋大学食品科技学院/国家贝类加工技术研发分中心(湛江)/广东省水产品加工与安全重点实验室/广东省海洋生物制品工程实验室/水产品深加工广东普通高等学校重点实验室,广东湛江524088 [2]暨南大学食品科学与工程系,广东广州510632 [3]大连工业大学海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034

出　　处：《南方水产科学》2022年第5期145-152,共8页South China Fisheries Science

基　　金：广东海洋大学博士启动项目(R17082);广东海洋大学创新强校专项项目(Q18292);2021年度省科技专项资金“大专项+任务清单”项目(2021A05197);现代农业产业技术体系资助(CARS-49);广东省现代农业产业技术体系创新团队项目(2021 KJ146);湛江市科技计划项目(2014C01019)。

摘　　要：为挖掘贝类活性肽潜在的营养功能价值,选用复合蛋白酶和风味酶分别水解马氏珠母贝(Pinctada martensii)、华贵栉孔扇贝(Chlamys nobilis)和栉江珧(Atrina pectinate)的闭壳肌,以α-葡萄糖苷酶抑制活性为主要考察指标,探讨了蛋白酶种类、酶解时间、pH、温度以及酶添加量对酶解产物α-葡萄糖苷酶抑制活性的影响,筛选出最佳酶解产物,通过动物实验进一步验证其辅助降血糖的活性。结果显示,3种贝类的酶解产物均具有良好的α-葡萄糖苷酶抑制活性,其中,马氏珠母贝闭壳肌酶解物(Enzymatic hydrolysate of P.martensii adductor muscle,EHPA)活性最强,在酶解条件时间为3 h、酶添加量为5000 U·g^(−1)、酶解温度为50℃、酶解pH为7.0的条件下,EHPA的α-葡萄糖苷酶抑制活性为24.54%,其抑制活性与短肽浓度有一定相关性。动物实验结果显示,EHPA具有辅助降血糖的活性。综上,EHPA具有开发辅助降血糖功能食品的潜在应用价值。To explore the potential nutritional value of shellfish active peptides,we hydrolyzed the adductor muscles of Pinctada martensii,Chlamys nobilis and Atrina pectinate by compound protease and flavor protease,and investigated the effects of protease types,enzymolysis time,pH,temperature and enzyme dosage of the enzymatic hydrolysate on the inhibitory activity onα-glucosidase,so as to screen out the optimal enzymatic hydrolysate and further verify its auxiliary hypoglycemic activity through animal experiments.Results show that the hydrolysate of three kinds of adductor muscle hadα-glucosidase inhibitory activity.The enzymatic hydrolysate of P.martensii adductor muscle(EHPA)was most active with enzymolysis time of 3 h,pH of 7.0,enzymolysis temperature of 50℃and enzyme dosage of 5000 U·g^(−1).Under these conditions,the inhibitory activity ofαglucosidase was 24.54%,which was related to the content of small molecular pepride.The results of animal experiments indicate that EHPA had the activity of auxiliary hypoglycemia.In summary,EHPA has potential application value in developing functional food for auxiliary hypoglycemic function.

关 键 词：马氏珠母贝 活性肽 降血糖 α-葡萄糖苷酶抑制活性 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]