高压微射流均质法制备二十二碳六烯酸藻油脂质体及其性质分析  

Preparation and Characterization of Docosahexaenoic Acid Algae-Rich Oil Liposomes by High-Pressure Microfluidizer Homogenization

作　　者：李畅 薛璐[1] 芦晶[2] 逄晓阳[2] 张书文[2] 吕加平[2] LI Chang;XUE Lu;LU Jing;PANG Xiaoyang;ZHANG Shuwen;Lü Jiaping(School of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China;Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]天津商业大学生物技术与食品科学学院,天津300134 [2]中国农业科学院农产品加工研究所,北京100193

出　　处：《食品科学》2022年第19期110-117,共8页Food Science

基　　金：黑龙江省“百千万”工程科技重大专项(2019ZX07B01)。

摘　　要：本实验通过高压微射流均质法制备二十二碳六烯酸(docosahexaenoic acid,DHA)藻油脂质体,以平均粒径、包封率为主要评价指标,研究大豆磷脂与胆固醇质量比、吐温-80用量、高压微射流均质压力等因素对DHA藻油脂质体的影响。在单因素试验基础上,通过响应面优化试验确定最佳制备工艺参数:大豆磷脂质量浓度为20 mg/m L,大豆磷脂与DHA藻油质量比为4∶1,大豆磷脂与胆固醇质量比为11.9∶1,吐温-80用量为大豆磷脂质量的15%,高压微射流均质压力为138 MPa,均质次数5次。在此条件下,DHA藻油脂质体平均粒径为(59.35±3.05)nm,多分散指数为0.189±0.025,包封率为(94.2±2.9)%。此外,对DHA藻油脂质体的理化性质进行了分析,通过透射电子显微镜观察发现DHA藻油脂质体的微观结构为球状,且分布均匀;通过差示扫描量热法分析发现,与未经高压微射流均质处理脂质体相比,高压微射流均质处理有效地提高了脂质体的相变温度;稳定性分析实验结果表明,经高压微射流均质处理过的DHA藻油脂质体具有良好的物理稳定性、贮藏稳定性及氧化稳定性。The effects of the mass ratio of soybean lecithin to cholesterol, the dosage of Tween-80 and homogenization pressure on the mean particle size and encapsulation efficiency of docosahexaenoic acid(DHA)-rich algae oil liposomes prepared by high-pressure microfluidization were studied. Using a combination of one-factor-at-a-time(OFAT) method and statistical response surface methodology(RSM), the optimal preparation parameters were determined as follows: soy lecithin concentration 20 mg/m L, lecithin to DHA algae oil mass ratio 4:1, lecithin to cholesterol mass ratio 11.9:1, Tween-80 dosage 15%(relative to soy lecithin), homogenization pressure 138 MPa, and five cycles of homogenization. The mean particle size,polydispersity index and encapsulation efficiency of the algal oil liposomes prepared under these conditions were(59.35 ±3.05) nm, 0.189 ± 0.025, and(94.2 ± 2.9)%, respectively. By transmission electron microscope(TEM), it was observed that the liposomes were spherical and uniformly distributed. Differential scanning calorimetric(DSC) analysis showed that highpressure microfluidizer homogenization improved the phase transition temperature of the liposomes effectively. Moreover,the liposomes treated with high-pressure microfluidizer homogenization had good physical, storage and oxidation stability.

关 键 词：二十二碳六烯酸藻油 脂质体 高压微射流均质 稳定性 

分 类 号：TS201.1[轻工技术与工程—食品科学]