高山被孢霉谷氨酸代谢调控产脂的初步研究  

Preliminary study on lipid production of Mortierella alpina regulated by glutamate metabolism

作　　者：蔡毅博 陈海琴[1] 张灏[1] 陈卫[1] CAI Yibo;CHEN Haiqin;ZHANG Hao;CHEN Wei(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学食品学院,江苏无锡214122

出　　处：《食品与发酵工业》2022年第20期188-195,共8页Food and Fermentation Industries

摘　　要：为解析谷氨酸代谢对产油丝状真菌高山被孢霉(Mortierella alpina)脂质合成的影响,该文考察了补加谷氨酸条件下高山被孢霉谷氨酸代谢相关酶的基因转录水平、酶活性水平和总脂产量等指标。结果显示,补加谷氨酸显著影响了高山被孢霉胞内谷氨酸代谢相关基因的转录水平,NADP+型谷氨酸脱氢酶和谷氨酸脱羧酶活性的增加为脂肪酸从头合成提供了充足的还原力,这使得细胞总脂含量提升了3 g/L(约占干重比例10%)。该研究为氨基酸介导的微生物脂质合成机制解析提供了参考。This study aimed to analyze the effect of glutamate metabolism on lipid synthesis in the oleaginous filamentous fungus Mortierella alpina.The gene transcription level and enzyme activity of glutamate metabolism-related enzymes and total lipid production in M.alpina under the condition of glutamate supplementation were measured.The results showed that glutamate supplementation significantly regulated the transcription level of glutamate metabolism-related genes in M.alpina.The increased activities of NADP+glutamate dehydrogenase and glutamate decarboxylase provided sufficient NADPH for the de novo synthesis of fatty acids,which increased the total cell lipid content by 3 g/L(about 10%of the dry weight).This study provides a reference for the analysis of amino acid-mediated microbial lipid synthesis mechanism.

关 键 词：高山被孢霉 谷氨酸代谢 脂质 转录 谷氨酸脱氢酶 谷氨酸脱羧酶 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]