酿酒酵母在糖质燃料乙醇中的发酵工艺优化  

Optimization of fermentation process for saccharide-based fuel ethanol production by Saccharomyces cerevisiae

作　　者：代勇华 刘盼婷 宋超 胡国全[1,3] 何明雄 DAI Yonghua;LIU Panting;SONG Chao;HU Guoquan;HE Mingxiong(Biomass Energy Technology Research Centre,Biogas Institute of Ministry of Agriculture and Rural Affairs,Chengdu 610041,China;Graduate School of Chinese Academy of Agricultural Science,Beijing 100081,China;Laboratory of Development and Application of Renewable Energy,Ministry of Agriculture and Rural Affairs,Chengdu 610041,China;Chengdu National Agricultural Science and Technology Center,Chengdu 610000,China)

机构地区：[1]农业农村部沼气科学研究所,生物质能技术研究中心,成都610041 [2]中国农业科学院研究生院,北京100081 [3]农业农村部可再生能源开发与利用重点实验室,成都610041 [4]成都市国家农业科技中心,成都610000

出　　处：《应用与环境生物学报》2022年第4期1076-1083,共8页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(32070036);中国农业科学院青年英才计划和基础研究计划项目资助。

摘　　要：低成本、高产量的发酵工艺是实现工业燃料乙醇经济和环境可持续性发展的关键,而不需要重大基础设施改变或投资.为获得酿酒酵母(Saccharomyces cerevisiae)利用甘蔗汁生产燃料乙醇的最优发酵工艺,首先对发酵体系的氮源条件进行优化;其次,在单因素试验基础上,以乙醇发酵效率为响应值,通过响应面法优化了燃料乙醇生产的发酵工艺,并通过补料分批发酵技术在5 L发酵罐中进一步扩大发酵.结果表明,以1.0 g/L (NH)SO和1.0 g/L酵母提取物作为发酵氮源,乙醇发酵效率和得率比对照可分别提高4.80%、9.52%.响应面设计获得的最优发酵工艺条件为在总糖浓度150.0 g/L、酵母提取物浓度2.0 g/L、发酵时间24.5 h、pH5.0、外加(NH)SO浓度1.0 g/L时,最高乙醇发酵效率可达到91.10%.在5 L发酵罐中采用补料分批发酵获得的最终乙醇浓度达到98.92 g/L,发酵效率维持在90%左右,乙醇生产力最高达到3.81 g Lh.本研究获得了一种高效生产糖质燃料乙醇的发酵工艺,可在较短时间内获得高浓度乙醇且消耗较少氮源,结果可为进一步利用糖质原料进行高效生物炼制及高浓度乙醇工业化生产提供参考.(图6表6参30)An efficient fermentation process is key to achieving economic and environmental sustainability of industrial fuel ethanol without requiring major infrastructure changes or investments. To obtain the optimal fermentation process for Saccharomyces cerevisiae to use sugarcane juice to produce fuel ethanol, the nitrogen source of the fermentation medium was first optimized. Response surface methodology was employed to optimize the fermentation process for fuel ethanol production based on single-factor experiments, using ethanol fermentation efficiency as an indicator. Fermentation was further expanded in a 5 L fermenter through fed-batch fermentation. The results showed that using 1.0 g/L(NH)SOand 1.0 g/L yeast extract as the nitrogen source for fermentation, the efficiency and yield of ethanol fermentation could be increased by 4.80%and 9.52%, respectively, compared to the control. The optimal fermentation process conditions obtained by response surface methodology are total sugar 150.0 g/L, yeast extract 2.0 g/L, fermentation time 24.5 h, pH 5.0,and additional(NH)SO1.0 g/L;in this situation, the maximum fermentation efficiency of ethanol can reach 91.10%. The final ethanol concentration obtained by fed-batch fermentation in the 5 L fermenter reached 98.92g/L, the fermentation efficiency was maintained at approximately 90%, and the ethanol productivity reached its highest at 3.81 g Lh. These results indicate that this study has obtained a fermentation process for the efficient production of sugar-based fuel ethanol, which can yield high-titer ethanol in a short time and consume less nitrogen. In addition, our research provides insights into the improvement of the bioconversion efficiency of saccharide feedstocks and industrial high-titer fuel ethanol production.

关 键 词：燃料乙醇 酿酒酵母 甘蔗 响应面法 补料分批发酵 

分 类 号：TQ920.1[轻工技术与工程—发酵工程] TQ223.122