基于纯培养和高通量测序技术解析仙桃地区鲊菜细菌多样性  

Analysis of bacterial diversity in Zha-vegetable from Xiantao region based on pure culture and high-throughput sequencing

作　　者：胡雨婷 符漫 王玉荣[1,2] 张彦 张海波[1,3] 孙雅芳[3] 郭壮[1,2] HU Yuting;FU Man;WANG Yurong;ZHANG Yan;ZHANG Haibo;SUN Yafang;GUO Zhuang(Hubei Provincial Engineering and Technology Research Center for Food Ingredients,Hubei University of Arts and Science,Xiangyang 441053,China;Xiangyang Lactic Acid Bacteria Biotechnology and Engineering Key Laboratory,Hubei University of Arts and Science,Xiangyang 441053,China;Angel Yeast Co.,Ltd.,Yichang 443003,China)

机构地区：[1]湖北文理学院湖北省食品配料工程技术研究中心,湖北襄阳441053 [2]湖北文理学院乳酸菌生物技术与工程襄阳市重点实验室,湖北襄阳441053 [3]安琪酵母股份有限公司,湖北宜昌443003

出　　处：《中国酿造》2022年第10期95-99,共5页China Brewing

基　　金：襄阳市基础研究类科技计划项目(2021ABS002937);湖北文理学院教师科研能力培育基金“科技创新团队”(2020kypytd009)。

摘　　要：为解析湖北省仙桃地区鲊菜中细菌多样性,该研究在采用纯培养技术对乳酸菌菌株进行分离鉴定的基础上,进一步使用Illumina MiSeq高通量测序技术对其细菌多样性进行解析。纯培养结果发现,分离到的44株乳酸菌隶属于乳酸杆菌属(Lactobacillus)、明串珠菌属(Leuconostoc)、魏斯氏菌属(Weissella)和乳球菌属(Lactococcus),其中植物乳杆菌(L.plantarum)和戊糖乳杆菌(L.pentosus)分别占总分离株含量的40.91%和34.09%。Illumina MiSeq高通量测序结果显示,鲊菜中优势细菌属主要包括乳酸杆菌属、明串珠菌属、魏斯氏菌属、假单胞菌属(Pseudomonas)和肠杆菌属(Enterobacter),其平均相对含量分别为85.03%、3.31%、1.68%、1.50%和1.05%。由此可见,仙桃地区鲊菜中乳酸菌具有较高的多样性,且以植物乳杆菌和戊糖乳杆菌为主。In order to analyze the bacterial diversity of Zha-vegetable from the Xiantao region of Hubei Province,on the basis of isolation and identification of lactic acid bacteria by pure culture technology,bacterial diversity was analyzed by Illumina MiSeq high-throughput technology.The pure culture results showed that the 44 strains of lactic acid bacteria isolated belonged to Lactobacillus,Leuconostoc,Weissella and Lactococcus.Among them,the L.plantarum and L.pentosus accounted for 40.91%and 34.09%of the total isolates,respectively.The Illumina MiSeq high-throughput sequencing results showed that the dominant bacterial genera in Zha-vegetable mainly included Lactobacillus,Leuconostoc,Weissella,Pseudomonas and Enterobacter,with average relative contents of 85.03%,3.31%,1.68%,1.50%and 1.05%,respectively.Therefore,the lactic acid bacteria in Zha-vegetable from Xiantao region had higher diversity,and the L.plantarum and L.pentosus were the main species.

关 键 词：鲊菜 纯培养技术 高通量测序 细菌多样性 

分 类 号：TS201.1[轻工技术与工程—食品科学] TS255.5[轻工技术与工程—食品科学与工程]