野葛总黄酮的提取工艺优化及抗氧化活性研究  

Optimization of extraction process of total flavonoids from Pueraria lobata and its antioxidant activity

作　　者：龚频 翟鹏涛 张梦璇 陈雪峰 王小娟 陈福欣[3] GONG Pin;ZHAI Pengtao;ZHANG Mengxuan;CHEN Xuefeng;WANG Xiaojuan;CHEN Fuxin(School of Food and Bioengineering,Shaanxi University of Science and Technology,Xi'an 710021,China;Shaanxi Agricultural Products Technical Institute,Xi'an 710021,China;School of Chemical and Chemistry,Xi'an University of Science and Technology,Xi'an 710054,China)

机构地区：[1]陕西科技大学食品与生物工程学院,陕西西安710021 [2]陕西农产品加工技术研究院,陕西西安710021 [3]西安科技大学化学与化工学院,陕西西安710054

出　　处：《中国酿造》2022年第10期172-176,共5页China Brewing

基　　金：西安市未央区科研创新基金(202036);榆林市科技计划项目(201907);陕西省科技厅重点产业创新链(群)-农业领域(2021ZDLNY04-01);陕西省科技厅一般项目(2019GY-138,2020GY-236,2021NY-161)。

摘　　要：通过单因素试验及正交试验确定野葛总黄酮的最佳提取工艺,并采用紫外分光光度法对野葛总黄酮的1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力、总还原能力和羟基(OH)自由基清除能力进行测定。结果表明,野葛总黄酮的最佳提取工艺条件为:体积分数40%的乙醇、料液比1∶20(g∶mL)、80℃水浴提取2 h,提取2次。在此优化条件下,野葛总黄酮的提取率3.06%。抗氧化试验结果表明,野葛总黄酮质量浓度分别为0.8 mg/mL、0.4 mg/mL、0.8 mg/mL时,DPPH自由基清除能力、OH自由基清除能力、总还原能力(OD700 nm值)最大值分别为72.98%、65.38%、0.16,表明野葛中总黄酮具有一定的抗氧化能力,且在一定浓度范围内,浓度越高抗氧化能力越强。The optimal extraction process of total flavonoids from Pueraria lobata was determined by single factor tests and orthogonal tests,and the 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging ability,total reducing ability and hydroxyl(OH)radical scavenging ability of total flavonoids from P.lobata were determined by ultraviolet spectrophotometry.The results showed that the optimal extraction conditions of total flavonoids from P.lobata were as follows:ethanol 40%vol,solid and liquid ratio 1∶20(g∶ml),extraction in 80℃water bath for 2 h,and extraction 2 times.Under the optimal conditions,the extraction yield of total flavonoids from P.lobata was 3.06%.The results of antioxidant tests showed that when the mass concentrations of total flavonoids from P.lobata were 0.8 mg/ml,0.4 mg/ml,and 0.8 mg/ml,respectively,the maximum value of DPPH radical scavenging ability,OH radical scavenging ability,and total reducing ability(OD700 nm value)were 72.98%,65.38%,and 0.16,respectively,indicating that the total flavonoids in P.lobata had a certain antioxidant ability,and within a certain concentration range,the higher the concentration,the stronger the antioxidant capacity.

关 键 词：野葛 总黄酮 提取工艺优化 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]