液相色谱质谱联用测定中毒样品中河豚毒素  

Determination of Tetrodotoxin in Poisoned Samples by Liquid Chromatography-Mass Spectrometry

作　　者：高榕冬 孙磊龙 章敏敏 彭骢 GAO Rongdong;SUN Leilong;ZHANG Minmin;PENG Cong(Jieyang Center for Disease Control and Prevention,Jieyang 522000,China)

机构地区：[1]揭阳市疾病预防控制中心,广东揭阳522000

出　　处：《食品安全导刊》2022年第24期101-105,共5页China Food Safety Magazine

基　　金：揭阳市科学技术局“多种有毒生物中的生物毒素检验方法建立”(ylxm003)。

摘　　要：建立血液、尿液、胃液以及鱼样中河豚毒素的液相色谱质谱联用测定方法。鱼样基质样品采用1%甲酸甲醇溶液去蛋白,上清液采用固相萃取法去除杂质,血液、胃液用乙腈提取,尿液静置沉淀,并均采用液相色谱质谱联用仪进行河豚毒素测定。实验结果表明,在线性范围内河豚毒素含量和峰面积具有良好的线性关系。本文所提出的测定中毒样品中河豚毒素的方法具有快速、高效、准确等优点,能够为河豚毒素中毒临床诊断、食品安全检测提供技术保障。A method for the determination of tetrodotoxin in blood,urine,gastric juice and fish samples by liquid chromatography-mass spectrometry was developed.The matrix of fish sample was deproteinized by 1%formic acid methanol solution,the supernatant was extracted by solid phase extraction,the blood and gastric juice were extracted by acetonitrile,and the urine was deposited,tetrodotoxin was determined by liquid chromatography-mass spectrometry(LC-MS).The results showed that tetrodotoxin content and peak area had a good linear relationship in the linear range.The method is rapid,efficient and accurate,which can be used for clinical diagnosis and food safety detection of tetrodotoxin poisoning.

关 键 词：河豚毒素 液相色谱质谱联用 固相萃取 

分 类 号：R155.5[医药卫生—营养与食品卫生学] O657.63[医药卫生—公共卫生与预防医学]