黑木耳醇提物对高脂肥胖小鼠肝脏和肠道菌群的调节作用  

Regulatory Effect of Ethanol Extracts from Auricularia heimuer on Liver and Intestinal Flora in High Fat Diet-induced Obese Mice

作　　者：刘培培 武少兰 吴敏文 杨成龙[3] 江玉姬[1,4] 陈炳智[1,4] Liu Peipei;Wu Shaolan;Wu Minwen;Yang Chenglong;Jiang Yuji;Chen Bingzhi(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002;Fuzhou Agricultural Bureau,Fuzhou 350000;Institute of Agricultural Engineering Technology,Fujian of Academy of Agricultural Sciences,Fuzhou 350001;Mycological Research Center of Fujian Agricultural and Forestry University,Fuzhou 350002)

机构地区：[1]福建农林大学食品科学学院,福州350002 [2]福建省福州市农业局,福州350000 [3]福建省农业科学院农业工程技术研究所,福州350001 [4]福建农林大学菌物研究中心,福州350002

出　　处：《中国食品学报》2022年第10期161-170,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2019YFC1710501)。

摘　　要：目的:探究黑木耳醇提物(AHA)对高脂膳食小鼠肝脏及肠道菌群的影响。方法:采用高脂饮食建立肥胖小鼠模型,连续饲喂9周,比较AHA干预前、后小鼠体质量、体长等生理、生化指标的变化。采用苏木精-伊红(HE)染色法观察肝脏细胞的体积以及数量变化,采用16S rRNA基因测序分析技术分析黑木耳醇提物对小鼠肠道菌群的影响。结果:经AHA干预的小鼠体质量、附睾脂肪指数和皮下脂肪指数显著降低(P<0.05),Lee’s指数、血清GLU浓度、肝脏指数极显著降低(P<0.01),肝脏HDL-C浓度显著升高(P<0.05)。HE染色结果表明小鼠脂肪细胞变小,脂滴减小,细胞核逐渐清晰。高通量测序结果表明AHA可以调节肠道菌群的多样性和丰富度。结论:黑木耳醇提物具有预防营养型小鼠肥胖,抑制小鼠脂肪肝形成,改善肝脏组织,减轻病变程度以及调节肠道菌群紊乱的作用。Objective: The influence of ethanol extracts obtained from Auricularia heimuer fruit body(AHA) on the liver function and the diversity and structure of gut microbiota in high-fat diet mice were explored. Methods: The obese mice model was established by high-fat diet. After feeding for 9 weeks, the changes of physiological and biochemical indicators of mice such as body weight and body length were compared before and after AHA intervention, and the size and number of liver cells were observed by Hematoxylin-eosin(HE) staining. 16S rRNA gene sequencing was used to profile the diversity and structure of intestinal flora in the cecum samples. Results: AHA treatment group significantly decreased the body weight, epididymis fat and subcutaneous fat index of mice(P<0.05), significantly decreased Lee’s index, serum GLU and liver index(P<0.01) and increased the content of HDL-C in the liver(P<0.05). In AHA group, the adipocytes and lipid droplets in mice were observed to become smaller and the nucleus gradually became clear by HE staining. In addition, High throughput sequencing showed that AHA could regulate the diversity and richness of intestinal flora. Conclusion: AHA could significantly prevent nutritional obesity, inhibit fatty liver formation, improve liver tissue, reduce the degree of lesion and regulate the disorder of intestinal flora.

关 键 词：黑木耳提取物 肝脏 肠道菌群 高通量测序 

分 类 号：TS201.4[轻工技术与工程—食品科学]