体外胃肠道消化及跨膜转运对干腌火腿肽抗炎活性的影响  

Effects of Gastrointestinal Digestion In Vitro and Transmembrane Transport on Anti-Inflammatory Activity of Bioactive Peptides from Dry-Cured Ham

作　　者：邢路娟 任晓镤[2] 王紫旭 郝月静 张万刚[1] XING Lujuan;REN Xiaopu;WANG Zixu;HAO Yuejing;ZHANG Wangang(National Center of Meat Quality and Safety Control,College of Food Science Technology,Nanjing Agricultural University,Nanjing 210095,China;College of Food Science and Engineering,Tarim University,Alar 843300,China)

机构地区：[1]南京农业大学食品科学技术学院,国家肉品质量安全与工程技术研究中心,江苏南京210095 [2]塔里木大学食品科学与工程学院,新疆阿拉尔843300

出　　处：《肉类研究》2022年第10期1-7,共7页Meat Research

基　　金：国家自然科学基金青年科学基金项目(32001720);中央高校基本科研业务费专项(KYLH2022005);南京农业大学-塔里木大学科研联合基金项目(NNLH202205)。

摘　　要：以宣威火腿为原料提取火腿中生物活性肽,通过体外模拟胃肠道消化和Caco-2细胞单层膜转运实验,进一步分析火腿肽的序列及抗炎功能变化。结果表明:经模拟胃肠道消化后,宣威火腿肽的总体数量增加,其中1000 Da以下多肽的数量占比从40.18%增加到57.40%;模拟消化后火腿肽抑制RAW264.7巨噬细胞炎症因子分泌的作用增强,经Caco-2细胞跨膜转运后共检测到24条多肽序列,其中包括12条四肽和5条三肽,分子质量小于500 Da的小肽占62.50%;合成小肽PAG、LVG、LGV、PVL具有缓解巨噬细胞分泌NO、肿瘤坏死因子-α的功能。因此,模拟胃肠道消化可以改变火腿肽的组成,进而提升其抗炎活性;跨膜转运后的小肽具有缓解巨噬细胞炎症因子分泌的功能。The changes in the sequence and anti-inflammatory properties of bioactive peptides extracted from Xuanwei ham were analyzed after stimulated gastrointestinal digestion in vitro and transport across Caco-2 monolayers.The results showed that the total number of bioactive peptides increased after gastrointestinal digestion,and the proportion of peptides with a molecular mass below 1000 Da in the total number of peptides increased from 40.18%to 57.40%.The inhibitory effect of the peptides on the secretion of inflammatory cytokines in RAW264.7 macrophages was enhanced after simulated digestion.After transport across Caco-2 cells,a total of 24 peptides were detected,including 12 tetrapeptides and 5 tripeptides,of which 62.50%were peptides with a molecular mass less than 500 Da.The synthetic peptides PAG,LVG,LGV and PVL were found to be able to suppress the secretion of NO and tumor necrosis factorαin macrophages.In conclusion,simulated gastrointestinal digestion can change the composition of the peptides from ham and in turn increase the anti-inflammatory activity.In addition,the small peptides transported across Caco-2 cells can inhibit the secretion of inflammatory factors in RAW264.7 macrophages.

关 键 词：干腌火腿 生物活性肽 体外消化 跨膜转运 抗炎活性 

分 类 号：TS251.5[轻工技术与工程—农产品加工及贮藏工程]