高效液相色谱-柱后光化学衍生法检测青稞、豌豆中黄曲霉毒素B1含量  

Determination of Aflatoxin B1 in Barley and Pea by High Performance Liquid Chromatography-post-column Photochemical Derivatization

作　　者：乔燕燕 王平 冯声宝 QIAO Yanyan;WANG Ping;FENG Shengbao(Qinghai Huzhu Highland Barley Wine Co.,Ltd.,Huzhu 810599,Qinghai,China)

机构地区：[1]青海互助青稞酒股份有限公司质量部,青海互助810599 [2]青海互助青稞酒股份有限公司,青海互助810599

出　　处：《酿酒》2022年第6期72-74,共3页Liquor Making

摘　　要：建立了青稞、豌豆中黄曲霉毒素B_(1)的柱后光化学衍生-高效液相色谱荧光检测器测定方法。样品经甲醇-水溶液(70∶30,v/v)溶液匀质提取,过黄曲霉毒素B_(1)免疫亲和柱净化,以甲醇-乙腈(50+50,v/v):水为流动相,流速为1.0 mL/min,进样量为50μL,荧光检测器检测,激发波长为360 nm,发射波长为440 nm。以保留时间定性,外标法定量。结果表明:该方法线性范围为0.10~40.0 ng/mL,相关系数R^(2)=0.9997,回收率为92.1%~96.9%,RSD<3%,黄曲霉毒素B_(1)方法检出限为0.03μg/kg。该方法高效便捷、重现性好,为青稞、豌豆等粮食作物中黄曲霉毒素B_(1)的检测提供了准确可靠的依据。A post-column photochemical derivatization method for the determination of aflatoxin B_(1) in barley and peas by high performance liquid chromatography with fluorescence detector was established.The sample was homogenously extracted with methanol-water solution(70:30,v/v),and purified by aflatoxin B_(1) immunoaffinity column.The mobile phase was methanol-acetonitrile(50+50,v/v):water,and the flow rate was It was 1.0 mL/min,the injection volume was 50μL,the fluorescence detector was detected,the excitation wavelength was 360 nm,and the emission wavelength was 440nm.Qualitative by retention time,quantified by external standard method.The results showed that the linear range of the method was 0.10-40.0 ng/mL,the correlation coefficient R^(2)=0.9997,the recoveries were 92.1%~96.9%,the RSD was less than 3%,and the detection limit of aflatoxin B_(1) method was 0.03μg/kg.The method is efficient,convenient and reproducible,and provides an accurate and reliable basis for the detection of aflatoxin B_(1) in barley,peas and other food crops.

关 键 词：青稞 豌豆 黄曲霉毒素B1 柱后光化学衍生 高效液相色谱法 

分 类 号：TS261.21[轻工技术与工程—发酵工程] TS207.3[轻工技术与工程—食品科学与工程]