遗传算法优化酶辅助提取刺玫果多糖工艺及其抗氧化活性研究  

Optimization of enzyme-assisted extraction process of polysaccharide from Rosa davurica Pall with genetic algorithm and its antioxidant activity

作　　者：赵惠茹[1] 韦欣婷 王靓怡 胡海燕 李雪[1] 姚博星 ZHAO Huiru;WEI Xinting;WANG Liangyi;HU Haiyan;LI Xue;YAO Boxing(College of Pharmacy,Xi’an Medical University,Xi’an 710021)

机构地区：[1]西安医学院药学院,西安710021

出　　处：《中国食品添加剂》2022年第11期43-50,共8页China Food Additives

基　　金：药学省级重点学科建设项目(2016YXXK04);国家级大学生创新训练计划项目(201911840004);陕西省大学生创新训练计划项目(S202111840051);西安医学院大学生创新训练计划项目(121521051);西安医学院科技能力提升项目(KJTS20220069)。

摘　　要：以刺玫果为原料,采用酶辅助水提法提取刺玫果多糖,以加酶量、酶解时间、料液比为考察因素,多糖提取率为评价指标,采用单因素试验和Box-Behnken响应面设计建立数学模型,应用遗传算法对模型进行优化,得到提取工艺最佳条件,并对刺玫果多糖进行抗氧化活性初步研究。结果表明:酶辅助法提取刺玫果多糖的最佳工艺条件为加酶量1.37%,酶解时间71min,料液比1∶30g/mL。Box-Behnken响应面法和遗传算法联用时,刺玫果多糖提取率高,为16.38%。刺玫果多糖具有显著的自由基清除作用,但与抗坏血酸对比,其对DPPH自由基、ABTS~+自由基和羟自由基的清除率均略低。Rosa davurica Pall was used as raw material,and polysaccharide was extracted by cellulase assisted water extraction method. The enzyme dosage,enzymatic hydrolysis time and solid-liquid ratio were selected as investigation factors and the polysaccharide extraction rate was the evaluation index. The mathematical model was established by single factor test and Box-Behnken response surface design. The model was optimized by genetic algorithm to obtain the best extraction conditions,and antioxidant activity of polysaccharide from Rosa davurica Pall was studied. The results showed that the optimum extraction conditions were as follows :enzyme dosage 1.37%,enzymatic hydrolysis time 71min,and solid-liquid ratio 1∶30g/mL. When Box-Behnken response surface design method was combined with genetic algorithm,the extraction rate of polysaccharide from Rosa davurica Pall was as high as 16.38%. The polysaccharide from Rosa davurica Pall had significant free radical scavenging effect. Compared with ascorbic acid,it had slightly lower scavenging rates of DPPH free radical,ABTSfree radical and hydroxyl free radical.

关 键 词：刺玫果 多糖 纤维素酶 提取工艺 遗传算法 抗氧化活性 

分 类 号：T202.1[一般工业技术]