产甲壳素脱乙酰酶赖氨酸芽孢杆菌的筛选及产酶初探  

Screening and Enzymatic-producing Study of Chitin Deacetylase Producing Bacteria-Lysinibacillus sp.

作　　者：孟欣 艾国峰 姜国涛 谢克毅 姜爱莉[1] MENG Xin;AI Guofeng;JIANG Guotao;XIE Keyi;JIANG Aili(College of Life Sciences,Yantai University,Yantai 264005,China)

机构地区：[1]烟台大学生命科学学院,山东烟台264005

出　　处：《食品工业科技》2022年第24期139-144,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金(41906102);山东省自然科学基金(ZR2020MC038)。

摘　　要：为了采用生物法降解天然甲壳素,本研究以甲壳素为唯一碳源,从自然发酵的虾皮中筛选产甲壳素脱乙酰酶的菌株,通过显色平板初筛和产酶活性复筛,利用形态学特征和16S rRNA序列分析对菌株进行鉴定。结果表明,筛选获得甲壳素脱乙酰酶产生菌X4经过形态学特征和16S rRNA序列分析确定为赖氨酸芽孢杆菌(Lysinibacillus sp.)。对该菌株进行发酵培养,其产生的甲壳素脱乙酰酶酶活为8.210 U/mL,对甲壳素的脱乙酰度为8.642%,研究结果对甲壳素的绿色生物利用具有较大的意义。In order to biodegrade natural chitin,in this study,chitin was used as the sole carbon source,obtain chitin deacetylase strains which could produce chitosan from natural chitin by biological method.Results showed that,the strains were identified by color plate screening and enzyme activity rescreening,morphological characteristics and 16S rRNA sequence analysis.A bacterium X4 with higher activity of chitin deacetylase was obtained,and was identified as Lysinibacillus sp..The fermentation culture of the strain showed that the activity of chitin deacetylase produced by X4 could reached 8.210 U/mL,and the deacetylation degree of chitin was 8.642%.The results was valuable for the green biological utilization of chitin.

关 键 词：甲壳素脱乙酰酶 赖氨酸芽孢杆菌 酶活 脱乙酰度 菌株鉴定 

分 类 号：Q93-31[生物学—微生物学]