激活蛋白-1家族成员JunB、c-Maf、c-Fos、c-Jun在初诊多发性骨髓瘤中的表达及临床意义  

Expression and clinical significance of activator protein-1 family members JunB,c-Maf,c-Fos and c-Jun in newly diagnosed multiple myeloma

作　　者：燕法红 邱志远[1] 李乾鹏 高伟杰 曹荣旋 王宝宏[1] YAN Fahong;QIU Zhiyuan;LI Qianpeng;GAO Weijie;CAO Rongxuan;WANG Baohong(Department of Hematology,First Affiliated Hospital of Weifang Medical University/Weifang People′s Hospital,Weifang,Shandong 261041,China;Weifang Medical University,Weifang,Shandong 261053,China)

机构地区：[1]潍坊医学院第一附属医院/潍坊市人民医院血液内科,山东潍坊261041 [2]潍坊医学院,山东潍坊261053

出　　处：《国际检验医学杂志》2022年第23期2852-2856,2862,共6页International Journal of Laboratory Medicine

基　　金：山东省医药卫生科技发展计划项目(2017WS415)。

摘　　要：目的探讨激活蛋白-1(AP-1)家族成员JunB、c-Maf、c-Fos、c-Jun在初诊多发性骨髓瘤(MM)中的表达及临床意义。方法选取40例初诊MM患者,应用实时荧光定量PCR检测患者骨髓中JunB、c-Maf、c-Fos、c-Jun mRNA表达水平,比较不同性别、国际分期系统(ISS)分期的MM患者上述各项指标水平;分析MM患者JunB、c-Maf、c-Fos、c-Jun mRNA表达水平与临床指标[年龄、血清β_(2)-微球蛋白、血红蛋白、清蛋白(ALB)、球蛋白(GLO)、血肌酐、血钙、骨髓浆细胞比例]水平间的相关性。结果不同性别、不同ISS分期MM患者JunB、c-Maf、c-Fos、c-Jun mRNA表达水平比较,差异无统计学意义(P>0.05)。MM患者JunB mRNA表达水平与GLO水平呈正相关(r=0.320,P=0.044);c-Maf mRNA表达水平与GLO水平呈正相关(r=0.350,P=0.027),与ALB水平呈负相关(r=-0.316,P=0.047);c-Fos mRNA表达水平与血钙水平呈正相关(r=0.317,P=0.046);c-Jun mRNA表达水平与各项临床指标水平均无相关性(P>0.05)。结论AP-1家族成员JunB、c-Maf、c-Fos、c-Jun mRNA表达水平与MM患者性别、ISS分期无明显关系;而JunB、c-Maf mRNA表达水平与MM患者蛋白合成能力相关,c-Fos mRNA表达水平与MM患者溶骨程度相关,JunB、c-Fos、c-Maf在MM的发生及发展中可能发挥了一定作用。Objective To explore the expression and clinical significance of activator protein-1(AP-1)family members JunB,c-Maf,c-Fos and c-Jun in newly diagnosed multiple myeloma(MM).Methods Forty newly diagnosed MM patients were selected,and the expression levels of JunB,c-Maf,c-Fos and c-Jun mRNA in bone marrow of the patients were detected by real-time fluorescent quantitative PCR.The levels of the above indexes in MM patients with different genders and International Staging System(ISS)stages were compared.The correlation between JunB,c-Maf,c-Fos,c-Jun mRNA expression levels and clinical indicators[age,serum β_(2)-microglobulin,hemoglobin,albumin(ALB),globulin(GLO),serum creatinine,serum calcium,bone marrow plasma cell ratio]levels of MM patients was analyzed.Results There was no significant difference in the expression levels of JunB,c-Maf,c-Fos and c-Jun mRNA in MM patients with different genders and ISS stages(P>0.05).JunB mRNA expression level was positively correlated with GLO level in MM patients(r=0.320,P=0.044).c-Maf mRNA expression level was positively correlated with GLO level in MM patients(r=0.350,P=0.027),and negatively correlated with ALB level in MM patients(r=-0.316,P=0.047).c-Fos mRNA expression level was positively correlated with serum calcium level in MM patients(r=0.317,P=0.046).There was no correlation between c-Jun mRNA expression level and clinical indicators levels in MM patients(P>0.05).Conclusion The expression levels of JunB,c-Maf,c-Fos and c-Jun mRNA of AP-1 family members are not significantly correlated with gender and ISS stages of MM patients.The expression levels of JunB and c-Maf mRNA are correlated with the protein synthesis ability of MM patients,and the expression level of c-Fos mRNA is correlated with the degree of bone dissolution in MM patients.JunB,c-Fos and c-Maf may play a certain role in the occurrence and development of MM.

关 键 词：激活蛋白-1 JUNB C-MAF C-FOS C-JUN 多发性骨髓瘤 

分 类 号：R733.3[医药卫生—肿瘤]