湖北黄精叶片挥发油GC-MS分析及抗氧化活性测定  

GC-MS Analysis and Antioxidant Activities Determination of Volatile Oil from Leaves of Polygonatum zanlanscianense Pamp.

作　　者：宋歌 夏娇 肖强 SONG Ge;XIA Jiao;XIAO Qiang(School of Forestry and Horticulture,Hubei Minzu University,Enshi 445000,China;Hubei Key Laboratory of Biological Resources Protection and Utilization,Enshi 445000,China)

机构地区：[1]湖北民族大学林学园艺学院,湖北恩施445000 [2]生物资源保护与利用湖北省重点实验室,湖北恩施445000

出　　处：《湖北民族大学学报（自然科学版）》2022年第4期374-379,384,共7页Journal of Hubei Minzu University:Natural Science Edition

基　　金：国家自然科学基金项目(31260057);湖北省科技厅技术创新重大专项项目(2019ACA120).

摘　　要：为测定湖北黄精叶片挥发油成分及抗氧化活性,采用同时蒸馏萃取法和超临界CO_(2)萃取法提取挥发油,通过气相色谱-质谱联用仪(gas chromatography-mass spectrometry,GC-MS)分析挥发油成分,进一步通过测定2种挥发油对DPPH、ABTS、O_(2)^(-)和OH自由基的清除率评价其抗氧化活性.结果显示,同时蒸馏萃取法提取的挥发油共检测出12类26种化合物,主要成分为呋喃、酸和酚类化合物;超临界CO_(2)萃取法提取的挥发油共检测出9类19种化合物,主要成分为酸和醇类化合物.抗氧化活性测定结果表明,2种方法得到的湖北黄精叶片挥发油均具有一定的抗氧化活性;采用同时蒸馏萃取法提取的挥发油抗氧化活性较高.In order to determine the components and antioxidant activity of volatile oil from leaves of Polygonatum zanlanscianense Pamp.,the volatile oil was extracted by simultaneous distillation extraction and CO_(2)supercritical fluid extraction.The components of the two volatile oils were analyzed by GC-MS.The antioxidant activity of the two volatile oils was evaluated by measuring the scavenging rates of DPPH,ABTS,O_(2)^(-) and OH free radicals.The results showed that 26 compounds of 12 categories were detected in the volatile oil extracted by simultaneous distillation extraction,with the main components being furan,acid and phenolic compounds.19 compounds of 9 categories were detected in the volatile oil extracted by supercritical CO_(2)extraction,and the main components were acids and alcohols.The results of antioxidant activity assay showed that the volatile oil of Polygonatum zanlanscianense Pamp.obtained by the two methods had certain antioxidant activity,and the volatile oil extracted by simultaneous distillation extraction had higher antioxidant activity.

关 键 词：湖北黄精 挥发油 气相色谱-质谱 抗氧化活性 同时蒸馏萃取 超临界CO_(2)萃取 

分 类 号：R284.2[医药卫生—中药学]